Easily discriminate between differentiated and undifferentiated cells with this colorimetric alkaline phosphatase assay stain that turns pluripotent cells red

Overview

Easily visualize undifferentiated cells with alkaline phosphatase stains

When you need to know which cells are undifferentiated, such as for monitoring pluripotency status, performing quality control of stem cells, or measuring alkaline phosphatase (AP) activity in situ, SBI’s AP Staining Kits are specific, sensitive, and reliable tools that can provide answers in as little as fifteen minutes. Unlike other pluripotency markers that are only present in certain subsets of undifferentiated cells, AP is highly active in embryonic stem cells, embryonic germ cells, and induced pluripotent stem cells, making it a universal pluripotency marker.

The Red-Color Alkaline Phophatase Staining Kit is a histochemical assay for use on cells grown in tissue culture wells or dishes, and includes substrate and dye for colorimetric read-out of enzymatic activity. The AP-positive, undifferentiated stem cells stain red, with color developing rapidly. The stain does not fade and stained samples can be kept at 4°C for up to one week.

SBI’s AP Staining Kits are available as Blue-Color (Cat.# AP100B-1), Red-Color (Cat.# AP100R-1), or Dual-Color (includes both blue and red stains; Cat.# AP100D-1)

Visualize the difference

Figure 1. SBI’s AP Staining Kits can stain pluripotent cells either blue (Blue-Color AP Staining Kit, Cat.# AP100B-1) or red (Red-Color AP Staining Kit, Cat.# AP100R-1).

Figure 2. Easily distinguish pluripotent cells from differentiated cells with SBI’s AP Staining Kits. CitationsLiu, L, et al. (2017) Nano-on-micro fibrous extracellular matrices for scalable expansion of human ES/iPS cells. Biomaterials. 2017 Apr 1; 124:47-54. PM ID: 28187394Won, YH, et al. (2016) Elucidation of Relevant Neuroinflammation Mechanisms Using Gene Expression Profiling in Patients with Amyotrophic Lateral Sclerosis. PLoS ONE. 2016 Nov 4; 11(11):e0165290. PM ID: 27812125Li, J, et al. (2016) Culture substrates made of elastomeric micro-tripod arrays for long-term expansion of human pluripotent stem cells. Journal of Materials Chemistry B. 2016 Nov 1; 5:236-244. Link: Journal of Materials Chemistry BLiu, L, et al. (2014) Nanofibrous gelatin substrates for long-term expansion of human pluripotent stem cells. Biomaterials. 2014 Aug 1; 35(24):6259-67. PM ID: 24811263Search all Citations

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