Overview

Monitor NF-κB signaling in Jurkat cells

Speed your studies with this pre-built 293 TN-based cell line for monitoring NF-κB signaling in real time. We’ve already integrated an expression cassette that includes NF-κB-responsive transcriptional elements upstream of a minimal CMV promoter (mCMV)-GFP-luciferase cassette. Expression of GFP (up to 300-fold over background) and luciferase only occurs in the presence of active NF-κB signaling, enabling screening for genetic and/or small molecule inhibitors and activators of the NF-κB signaling pathway. Use GFP fluorescence for live cell imaging and FACS, or luciferase activity to quantitatively measure activity of the signaling pathway.

• Study NF-κB signaling in 293 TN cells
• Perform live cell imaging or FACS (300-fold over background NF-κB-dependent GFP signal)
• Measure pathway activity with luciferase
• Compatible with a variety of screening methods including small molecule and RNAi

Supporting Data

See the NF-κB/293/GFP-luc Transcriptional Reporter Cell Line in action

Monitor NF-κB in real time with GFP

Quantitate NF-κB Activation with luciferase assays

Resources

Citations

• Cen, X, et al. (2019) TLR1/2 Specific Small-Molecule Agonist Suppresses Leukemia Cancer Cell Growth by Stimulating Cytotoxic T Lymphocytes. Adv Sci (Weinh).2019 May 17; 6(10):1802042. PM ID:31131189
• Cen, X, et al. (2019) TLR1/2 Specific Small‐Molecule Agonist Suppresses Leukemia Cancer Cell Growth by Stimulating Cytotoxic T Lymphocytes. Adv. Sci..2019 Mar 27;:1802042. Link:Adv. Sci.
• Wang, J, et al. (2019) Siglec-15 as an immune suppressor and potential target for normalization cancer immunotherapy. Nat. Med..2019 Mar 4;. PM ID:30833750
• O"Grady, B, et al. (2019) Spatiotemporal control and modeling of morphogen delivery to induce gradient patterning of stem cell differentiation using fluidic channels. Biomater Sci.2019 Feb 19;. PM ID:30778445
• Ma, Y, et al. (2019) Inhibition of pancreatic cancer stem cell characteristics by α-Mangostin: Molecular mechanisms involving Sonic hedgehog and Nanog. J. Cell. Mol. Med..2019 Feb 3;. PM ID:30712329
• Yuan, S, et al. (2019) SREBP-dependent lipidomic reprogramming as a broad-spectrum antiviral target. Nat Commun.2019 Jan 10; 10(1):120. PM ID:30631056
• Lin, YS, et al. (2019) Liver X Receptor/Retinoid X Receptor Pathway Plays a Regulatory Role in Pacing-Induced Cardiomyopathy. J Am Heart Assoc.2019 Jan 8; 8(1):e009146. PM ID:30612502
• Heun, Y, et al. (2017) HIF-1α Dependent Wound Healing Angiogenesis In Vivo Can Be Controlled by Site-Specific Lentiviral Magnetic Targeting of SHP-2. Mol. Ther..2017 Jul 5; 25(7):1616-1627. PM ID:28434868
• Phua, KKL, Liu, Y & Sim, SH. (2017) Non-linear enhancement of mRNA delivery efficiencies by influenza A derived NS1 protein engendering host gene inhibition property. Biomaterials.2017 Jul 1; 133:29-36. PM ID:28426973
• Takada, S, et al. (2017) Pluripotent stem cell models of Blau syndrome reveal an IFN-γ-dependent inflammatory response in macrophages. J. Allergy Clin. Immunol..2017 Jun 3;. PM ID:28587749
• Li, X, et al. (2017) Nucleus-Translocated ACSS2 Promotes Gene Transcription for Lysosomal Biogenesis and Autophagy. Mol. Cell.2017 Jun 1; 66(5):684-697.e9. PM ID:28552616
• Chen, YC, et al. (2017) Selective Photomechanical Detachment and Retrieval of Divided Sister Cells from Enclosed Microfluidics for Downstream Analyses. ACS Nano.2017 May 23; 11(5):4660-4668. PM ID:28480715
• Choi, EJ, et al. (2017) A clinical drug library screen identifies clobetasol propionate as an NRF2 inhibitor with potential therapeutic efficacy in KEAP1 mutant lung cancer. Oncogene.2017 May 15;. PM ID:28504720
• Krabbe, G, et al. (2017) Microglial NFκB-TNFα hyperactivation induces obsessive-compulsive behavior in mouse models of progranulin-deficient frontotemporal dementia. Proc. Natl. Acad. Sci. U.S.A..2017 May 9; 114(19):5029-5034. PM ID:28438992
• Schmidt, C, et al. (2017) Pre-clinical drug screen reveals topotecan, actinomycin D and volasertib as potential new therapeutic candidates for ETMR brain tumor patients. Neuro-oncology.2017 May 8;. PM ID:28482026
• Pryke, KM, et al. (2017) A Novel Agonist of the TRIF Pathway Induces a Cellular State Refractory to Replication of Zika, Chikungunya, and Dengue Viruses. MBio.2017 May 2; 8(3). PM ID:28465426
• Gryder, BE, et al. (2017) PAX3-FOXO1 Establishes Myogenic Super Enhancers and Confers BET Bromodomain Vulnerability. Cancer Discov.2017 Apr 26;. PM ID:28446439
• Jin, C, et al. (2017) Over-expression of ASIC1a promotes proliferation via activation of the β-catenin/LEF-TCF axis and is associated with disease outcome in liver cancer. Oncotarget.2017 Apr 18; 8(16):25977-25988. PM ID:27462920
• Wang, W, et al. (2017) Biological or pharmacological activation of protein kinase C alpha constrains hepatitis E virus replication. Antiviral Res..2017 Apr 1; 140:1-12. PM ID:28077314
• Teng, CT, et al. (2017) Development of Novel Cell Lines for High-Throughput Screening to Detect Estrogen-Related Receptor Alpha Modulators. SLAS Discov.2017 Jan 1;:2472555216689772. PM ID:28346099