Overview
GainathoroughunderstandingofmiRfunction
PermanentlyknockdownmiRswithSBI’smiRZip™anti-miRNAExpressionLentivectors.LeveragingSBI’spowerfulandwell-regardedthird-generationlentivectortechnology,eachofourmiRZiplentivectorsexpressesashorthairpinRNA(shRNA)that,afterprocessing,preferentiallyproducesananti-sensemiRNA.Thehairpinisrationallydesignedtobeasymmetric,ensuringthatthesensestranddoesnotcontaintheendogenousmiRNAsequenceandenablingaccumulationoftheanti-miRNA.TheresultisrobustderepressionofthetranscriptstargetedbythemicroRNAbeing“zipped,”andelevationofthecorrespondingproteinlevels.
StudymiRswithSBI’spowerfulmiRZiptechnology:
- Stableandpermanentanti-microRNAexpressionfromaconstitutiveH1promoter
- Rationallydesigned,asymmetrichairpinsoptimizedforanti-sensemicroRNAproduction
- EfficientsuppressionofspecificendogenousmicroRNAs
- Reliabledeliverytodividingornon-dividingcells
- Selection/sortingfortransfected/transducedcellswitheithercopGFPorpuromycin
Conducthigh-throughputphenotypicscreenswithourpoolofpre-packagedlentiviralparticlescontainingourentiremiRZipcollection(Cat.#MZIPPLVA-1).
SupportingData
SeeSBI’smirZiptechnologyinaction
miRZipconstructsexpresshighlevelsofanti-microRNAs
Figure1.miRZipconstructsexpresshighlevelsofanti-microRNAs.miRZipconstructsweretransfectedintoHEK293cells,totalRNAisolated,andmiRZiplevelsmeasuredusingSBI’sQuantiMirAssay(Cat.#RA420A-1).ComparedtoQuantiMirassaysperformedonRNAisolatedfromnon-transfectedcells,themiRZip-transfectedcellsdeliveredhighexpressionlevelsofthespecificmiRZipanti-miRNAs.
EfficientmodulationoftargetproteinlevelsbySBI’sLenti-miRandmiRZiptechnologies
Figure2.TargetproteinlevelsareefficientlymodulatedbySBI’sLenti-miR-29aandmiRZip-29amicroRNAconstructs.
miRZiptechnologyiseffectiveinafunctionalassay
Figure3.miRZip-21andmiRZip-145exhibitanti-oncogenicandoncogenicactivity,respectively,inacellinvasionassayusingMDA-MB-231breastcancercells.(Toppanel)miR-21isanoncogenicmiRNAandmiR-145isatumorsuppressingmiRNA.TransductionofmiRZip-21(theanti-miR21lentiviralconstruct)andmiRZip-145(theanti-miR145lentiviralconstruct),inhibitedtherespectivemiRs,resultinginalowerpercentageofinvADIngcellsinmiRZip-21-transducedcellsandahigherpercentageofinvadingcellsinmiRZip-145-transducedcells.(Bottompanel)Additionally,themiRZip-145lentivirusinhibitedendogenousmiR-145andelevatedproteinexpressionlevelsofthemiR-145targetoncogenec-Myc.
AvailablemiRZip™anti-miRNAExpressionLentivectors
Usethesearchboxbelowtofindaproductbynameorcatalognumber
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Description
Size
CatalogNumber
Price
Quantity
AddtoCart
miRZip-1anti-miR-1microRNAconstruct
BacterialStreak
MZIP1-PA-1
$569
miRZip-100anti-miR-100microRNAconstruct
BacterialStreak
MZIP100-PA-1
$569
miRZip-101anti-miR-101microRNAconstruct
BacterialStreak
MZIP101-PA-1
$569
miRZip-103anti-miR-103microRNAconstruct
BacterialStreak
MZIP103-PA-1
$569
miRZip-106aanti-miR-106amicroRNAconstruct
BacterialStreak
MZIP106a-PA-1
$569
miRZip-106b+93+25Tripleanti-miR-106b+93+25microRNAconstruct
BacterialStreak
MZIP106b-93-25p-PA-1
$569
miRZip-106banti-miR-106bmicroRNAconstruct
BacterialStreak
MZIP106b-PA-1
$569
miRZip-107anti-miR-107microRNAconstruct
BacterialStreak
MZIP107-PA-1
$569
miRZip-10aanti-miR-10amicroRNAconstruct
BacterialStreak
MZIP10a-PA-1
$569
miRZip-10banti-miR-10bmicroRNAconstruct
BacterialStreak
MZIP10b-PA-1
$569
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SBI ExoQuick PLUS 系列
高纯度 Exosome 分离—满足敏感下游实验
ExoQuick PLUS 和 ExoQuick-TC PLUS 试 剂 盒 中 包 含 ExoQuick 或ExoQuick-TC,以及可直接使用的预包被特制微珠。在使用 ExoQuick 系列试剂提取出外泌体沉淀后,仅再需 10 分钟操作,即可获得高纯度的完整活性 exosomes。
产品特色
Cleaner — 与柱式法血清外泌体提取试剂盒相比,最多可减少 85% 的血清白蛋白和免疫球蛋白
Better — 与柱式法或试剂沉淀法相比,最多可减少 50% 的杂蛋白残留
Powerful — 最多可 3 倍提高 biomarker 检测效率
Fast — 在 ExoQuick 试剂提取后,仅需 10 分钟手动操作即可
试剂盒组分
ExoQuick PLUS 试剂盒
ExoQuick Reagent
2 mL
ExoQuick PLUS Microsphere Beads
10 units
Resuspension Buffer
15 mL
注:试剂盒中 ExoQuick PLUS Microsphere Beads,每个单位基于 250μL血清样本。如果是血浆样本,则需另外购买凝血酶素(货号 TMEXO-1)预处理后,再搭配此试剂盒使用。
ExoQuick-TC PLUS 试剂盒
ExoQuick-TC Reagent
10 mL
ExoQuick PLUS Microsphere beads
10 units
Resuspension Buffer
15 mL
注:试剂盒中 ExoQuick PLUS Microsphere Beads,每个单位基于适 5mL细胞培养样本。
产品信息
货号
产品描述
规格
说明
PM-EQPL10A-1
ExoQuick PLUS Exosome Purification Kit
10 rxns
以 250uL 血清起始样本为 1 次反应血浆样本,需要另外购买凝血酶素(货号 TMEXO-1)处理后,再用此试剂盒提取。
PM-EQPL10TC-1
ExoQuick-TC PLUS Exosome Purification Kit
10 rxns
以 5mL 细胞培养上清为 1 次反应