Study the balance of T cell dynamics in autoimmunity, inflammation, and cancer

Simplify your studies of T cell dynamics with SBI’s ready-to-use, pre-built Treg and Th17 vectors and cell lines. With pMSCV-Mouse Foxp3-EF1α-GFP-T2A-Puro for Foxp3 Overexpression, you can choose between three different ways to study Foxp3 overexpression from the MSCV promoter for strong expression in hematopoietic and stem cells:

• Lentivector-based for transfection-resistant cell lines

Create stable Foxp3-overexpressing cell lines by integrating the MSCV-Mouse Foxp3-EF1α-GFP-T2A-Puro expression cassette into the genome using SBI’s highly regarded and reliable lentivector technology. With our lentivector technology, you can take advantage of our high-titer lentivector packaging system to efficiently deliver your Foxp3 overexpression cassette to cells using transduction—great for transfection-resistant cell lines.

• PiggyBac Transposon-based* for infection-resistant cell lines

Create stable Foxp3-overexpressing cell lines by integrating the MSCV-Mouse Foxp3-EF1α-GFP-T2A-Puro expression cassette into the genome with SBI’s easy and consistent PiggyBac Transposon System. With our PiggyBac Transposon System, you get easy and consistent transgenesis for Foxp3 overexpression in infection-resistant cell lines.

• Pre-built Jurkat cell line to save time and labor

Quickly get your Treg studies off the ground with our pre-built Jurkat cell line that already has the MSCV-Mouse Foxp3-EF1α-GFP-T2A-Puro expression cassette integrated into the genome. We transduced Jurkat cells with the MSCV-Mouse Foxp3-EF1α-GFP-T2A-Puro expression cassette and have validated Foxp3 overexpression, so you don’t have to—just culture cells and start your studies.

*Customer Agreements

Academic customers can purchase PiggyBac Transposon System components for internal research purposes for indefinite use, whereas commercial customers must sign a customer agreement for a six-month, limited-use license to test the technology.

For end user license information, see the following:

• Academic Customers—End-User License Information
• Commercial Customers—End-User License Information

* SBI is fully licensed to distribute PiggyBac vectors as a partnership with Transposagen Biopharmaceuticals, Inc.

SBI ExoQuick PLUS 系列 高纯度 Exosome 分离—满足敏感下游实验 ExoQuick PLUS 和 ExoQuick-TC PLUS 试 剂 盒 中 包 含 ExoQuick 或ExoQuick-TC，以及可直接使用的预包被特制微珠。在使用 ExoQuick 系列试剂提取出外泌体沉淀后，仅再需 10 分钟操作，即可获得高纯度的完整活性 exosomes。 产品特色 Cleaner — 与柱式法血清外泌体提取试剂盒相比，最多可减少 85% 的血清白蛋白和免疫球蛋白 Better — 与柱式法或试剂沉淀法相比，最多可减少 50% 的杂蛋白残留 Powerful — 最多可 3 倍提高 biomarker 检测效率 Fast — 在 ExoQuick 试剂提取后，仅需 10 分钟手动操作即可 试剂盒组分 ExoQuick PLUS 试剂盒 ExoQuick Reagent 2 mL ExoQuick PLUS Microsphere Beads 10 units Resuspension Buffer 15 mL 注：试剂盒中 ExoQuick PLUS Microsphere Beads，每个单位基于 250μL血清样本。如果是血浆样本，则需另外购买凝血酶素（货号 TMEXO-1）预处理后，再搭配此试剂盒使用。   ExoQuick-TC PLUS 试剂盒 ExoQuick-TC Reagent 10 mL ExoQuick PLUS Microsphere beads 10 units Resuspension Buffer 15 mL 注：试剂盒中 ExoQuick PLUS Microsphere Beads，每个单位基于适 5mL细胞培养样本。 产品信息 货号 产品描述 规格 说明 PM-EQPL10A-1 ExoQuick PLUS Exosome Purification Kit 10 rxns 以 250uL 血清起始样本为 1 次反应血浆样本，需要另外购买凝血酶素（货号 TMEXO-1）处理后，再用此试剂盒提取。 PM-EQPL10TC-1 ExoQuick-TC PLUS Exosome Purification Kit 10 rxns 以 5mL 细胞培养上清为 1 次反应