Monitor and measure the expression of pluripotency markers

With SBI’s line of pGreenFire Pluripotency Response Reporter Vectors, you can monitor and measure the expression of pluripotency markers in live cells in real time. These vectors leverage our reliable lentivector technology and save you time—the pre-built reporters come as ready-to-package lentivector plasmids, with some reporters also available as ready-to-transduce, pre-packaged virus. All of our Response Reporters deliver great performance in both human and mouse cells.

The Sox2 SRR2 pGreenFire (EF1α-puro) Response Reporter uses the Sox2-responsive SRR2 enhancer with a mouse CMV promoter to co-express GFP and luciferase. Co-expression is mediated by a T2 element. The minimal CMV promoter alone delivers negligible expression, but when downstream of SSR2 enhancer elements, drives expression of dscGFP and luciferase under conditions where Sox2 is also expressed. The result is the ability to quantitatively measure Sox2 expression by fluorescence and luciferase activity.

The vector also includes a constitutively-expressed puromycin marker (EF1α promoter) to simplify cell line construction. Or choose a constitutively expressed neomycin marker (Cat.# SR20071-PA-N), or no marker at all (Cat.# SR20071-PA-1).

• Create stable cell lines for quantitation of Oct4 expression
• Track differentiation in live cells in real time

Please note that these vectors only function properly when transduced. Transfection keeps the constitutive RSV promoter intact, leading to nonspecific expression of the reporter genes.

Choose the right vector to monitor pluripotency markers (Pluripotency Reporters) or to measure the activity of pluripotency makers (Pluripotency Response Reporters)

Pluripotency response reporters

SBI’s Pluripotency Response Reporters work for both human and mouse stem cells
Catalog #	Promoter/Enhancer elements	Reporter	Selection cassette
SR20070-PA/VA-1	Oct4 CR4	pGreenFire (GFP-Luc)	None
SR20070-PA-N	Oct4 CR4	pGreenFire (GFP-Luc)	EF1α-Neo
SR20070-PA-P	Oct4 CR4	pGreenFire (GFP-Luc)	EF1α-Puro
SR20071-PA/VA-1	Sox2 SRR2	pGreenFire (GFP-Luc)	None
SR20071-PA-N	Sox2 SRR2	pGreenFire (GFP-Luc)	EF1α-Neo
SR20071-PA/VA-P	Sox2 SRR2	pGreenFire (GFP-Luc)	EF1α-Puro

Pluripotency reporters

Catalog #	Species	Promoter/enhancer element	Reporter
SR10033PA/VA-1	Human	Oct-4	pGreenZeo
SR10043PA/VA-1	Human	Oct-4	pRedZeo
SR10053PA/VA-1	Human	Oct-4	pRedTK
SR10029PA/VA-1	Mouse	Oct-4	pGreenZeo
SR10045PA/VA-1	Mouse	Oct-4	pRedZeo
SR10054PA/VA-1	Mouse	Oct-4	pRedTK
SR10030PA/VA-1	Human	Nanog	pGreenZeo
SR10042PA/VA-1	Human	Nanog	pRedZeo
SR10055PA/VA-1	Human	Nanog	pRedTK
SR10031PA/VA-1	Mouse	Nanog	pGreenZeo
SR10044PA/VA-1	Mouse	Nanog	pRedZeo
SR10056PA/VA-1	Mouse	Nanog	pRedTK

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