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Streamline your exosome detection with Exo-Check Arrays
For the most efficient detection of exosomes, turn to our semi-quantitative Exo-Check Exosome Antibody Arrays. Each array has 12 pre-printed spots and features 8 antibodies for known exosome markers (CD63, CD81, ALIX, FLOT1, ICAM1, EpCam, ANXA5 and TSG101), a GM130 cis-Golgi marker to monitor any cellular contamination in your exosome isolations, a labeled positive control for HRP detection, and a blank spot as a background control. The kits come complete with a secondary detection mixture conjugated to HRP.
1 | 2 | 3 | 4 | 5 | 6 | |
---|---|---|---|---|---|---|
A | CD63 | EpCam | ANXA5 | TSG101 | Blank | Positive control |
B | Positive control | GM130 | FLOT-1 | ICAM | ALIX | CD81 |
Spot position* | ID | Notes | Link to ExoCarta entry for the human protein |
---|---|---|---|
A1 | CD63 | Tetraspanin | ExoCarta_967 |
A2 | EpCam | Epithelial cell adhesion molecule; often found in cancer-derived exosomes | ExoCarta_4072 |
A3 | ANXA5 | Annexin A5 | ExoCarta_308 |
A4 | TSG101 | Tumor susceptibility gene 101 | ExoCarta_7251 |
A5 | Blank | Background control | NA |
A6 | Positive control | Positive control for HRP detection (derived from human serum exosomes) | NA |
B1 | Positive control | Labeled positive control for HRP detection | NA |
B2 | GM130 | Cis-golgi matrix protein—control for cellular contamination in exosome preparation | NA |
B3 | FLOT1 | Flotillin-1 | ExoCarta_10211 |
B4 | ICAM1 | Intercellular adhesion molecule 1 | ExoCarta_3383 |
B5 | ALIX | Programmed cell death 6 interacting protein (PDCD6IP) | ExoCarta_10015 |
B6 | CD81 | Tetraspanin | ExoCarta_93185 |
*To correctly orient the array, place the notched corner in the upper left-hand position |
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