扫码解锁，免费下载文件 请使用最新版本仪器信息网APP扫码登录 步骤：打开仪器信息网APP并登录 ——》点击APP首页右上角扫一扫 ——》APP端确认解锁 ——》PC端下载文件 免费注册PurposeThis kit allows for the determination of DNMT concentrations in Rat serum, cell culture supernates and other bi简介：Rat DNMTFOR RESEARCH USE ONLYAssay range：7 pg/ml - 260pg/ml 96 determinationsPurposeThis kit allows for the determination of DNMT concentrations in Rat serum, cell culture supernates and other biological fluidsPrinciple of the assayThe kit assay Rat DNMT level in the sample，use Purified Rat DNMT antibody to coat microtiter plate wells, make solid-phase antibody, then add DNMT to wells, Combined DNMT antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat DNMT in the samples is then determined by comparing the O.D. of the samples to the standard curve. 详细>

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