This affinity-purified antipeptide antibody was generated in chickens against a sequence shared between the mouse (CAA01912) and human (NP_000808) gene products.
Volume: 1000 µL
Concentration: 100 µg/mL
Clonality: Polyclonal
Host Species: Chicken
Species Reactivity:Human, Mouse, Rat
Applications: ICC, IHC, WB
Protein Name / Synonyms: Glutamate decarboxylase 1 (EC 4.1.1.15) (67 kDa glutamic acid decarboxylase) (GAD-67) (Glutamate decarboxylase 67 kDa isoform)
Target Description: Human Glutamic Acid Decarboxylase (GAD-67), [EC 4.1.1.15] is a 66,987 dalton protein (594 amino acids) selectively expressed in a subpopulation of GABAergic neurons of the CNS.It catalyzes the decarboxylation of glutamic acid, forming the inhibitory neurotransmitter beta-amino butyric acid (GABA). It is also know as GAD-1.
Gene ID: GAD1 GAD GAD67
Antibody Registry ID (RRID): AB_2313545
Physical State: Liquid
Production Notes: Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. This synthetic peptide corresponded to a region near the Cterminus of this gene product, and was 100% conserved between the human (Q99259, NCBI), mouse (P48318, NCBI) and rat (NP_058703, NCBI) gene products. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 100 µg/mL, and the preparation was filter-sterilized through a 0.45 µm filter.
Validation and Application Notes
Molecular Weight: 67 kDa
Western Blot Dilution Range: 1:1000-1:2000
IHC Dilution Range: 1:500-1:1000
Quality Control
This antibody preparation was analyzed by immunohisto-chemistry (at a concentration of 1 µg/mL) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Storage
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.
NOTE
Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.
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鸡的概念 在一次会议上，他与同事的一次谈话说服了西蒙博士再次尝试制造这种抗体，但是尝试把鸡作为宿主——鸡在进化上与包括人类在内的哺乳动物完全不同。结果表明:人蛋白抗原具有高度的免疫原性，抗体产量异常高，免疫试剂结合紧密，特异性高。 今天的Aves 从那时起，Aves Labs开创了在蛋白质中寻找高免疫原性肽序列的新算法，开发了针对鸡抗体的新的亲和纯化方法，并开发了许多其他有用的试剂，如BlokHen®阻断试剂和PrecipHen®免疫沉淀试剂。Aves实验室继续开发针对神经特异性抗原和表位标签的新型抗体试剂，以促进神经科学和分子生物学领域的科学进步。
