Details

ChickenswereimmunizedwithActinpurifiedfromrabbitmuscle.Afterrepeatedinjections,immuneeggswerecollected,andtheIgYfractionswerepurifiedfromtheyolks.TheseIgYfractionswerethenaffinity-purifiedusingapeptidecolumn.Thefinalproductcontains50%glycerol(v/v),purifiedanti-ActinIgYfraction(10mg/ml)spikedwith25µg/mlofaffinitypurifiedanti-ActinIgY,andthenfilter-sterilized.

Volume:100µL(ACT-1010)or400µL(ACT-1040)

Concentration:10mg/mL

Clonality:Polyclonal

HostSpecies:Chicken

SpeciesReactivity:Human,Mouse,Rat

Applications:WB

AntibodyRegistryID(RRID):AB_2313504

PhysicalState:Liquid

Buffer:Sodiumphosphate(10mM,pH7.2)bufferedisotonicsaline(0.9%,w/v),glycerol(50%,v/v),withsodiumazide(0.02%,w/v)asananti-microbialagent.

ValidationandApplicationNotes

MolecularWeight:42kDa

QualityControl

Thisanti-ActinIgYpreparationwasanalyzedbyWesternBlotanalysis(atadilutionof1:2000)usingHRP-labeledgoatanti-chickenIgY(1:5000dilution,AvesLabsCat.#H-1004)asthesecondaryreagent.

Storage

Storeat-20˚Cinthedark.Undertheseconditions,theantibodiesshouldhaveashelflifeofatleast12months(providedtheyremainsterile).Donotfreezeantibodiesat-80˚C,unlessyouwanttostorethemforlongerperiodsoftime.Notethateachtimeanantibodypreparationisfrozenabouthalfitsbindingactivityislost.

NOTE

AvesLabsproductsareintendedforuseasresearchlaboratoryreagents.Theyarenotintendedforuseasdiagnosticortherapeuticreagentsinhumans.

↑backtotop

鸡的概念 在一次会议上，他与同事的一次谈话说服了西蒙博士再次尝试制造这种抗体，但是尝试把鸡作为宿主——鸡在进化上与包括人类在内的哺乳动物完全不同。结果表明:人蛋白抗原具有高度的免疫原性，抗体产量异常高，免疫试剂结合紧密，特异性高。 今天的Aves 从那时起，Aves Labs开创了在蛋白质中寻找高免疫原性肽序列的新算法，开发了针对鸡抗体的新的亲和纯化方法，并开发了许多其他有用的试剂，如BlokHen®阻断试剂和PrecipHen®免疫沉淀试剂。Aves实验室继续开发针对神经特异性抗原和表位标签的新型抗体试剂，以促进神经科学和分子生物学领域的科学进步。