Our beta-galactosidase antibody recognizes the LacZ gene product with high titre and high specificity. Since this antibody was generated in chickens, it can be used together with your existing rabbit and mouse primary antibodies for double immunostaining with no secondary antibody cross reactivity. It even works well with fusion proteins of LacZ!

Volume: 100 µL (BGL-1010) or 400 µL (BGL-1040)

Concentration: 10 mg/mL

Clonality: Polyclonal

Form: Mixture of IgY fraction and affinity-purified antibodies

Host Species: Chicken

Applications: ICC, IHC, WB

Antibody Registry ID (RRID): AB_2313508

Physical State: Liquid

Production Notes: Chickens were immunized with purified ß-Galactosidase (lacZ) protein emulsified in Freund’s adjuvants. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using ß-Galactosidase conjugated to an agarose matrix. The final product is a mixture of both affinity-purified antibodies (25 µg/mL) and purified IgY (10 mg/mL), and is filter-sterilized.

Validation and Application Notes

Western Blot Dilution Range: 1:5000-1:10000

IHC Dilution Range: 1:2500-1:5000

Quality Control

This antibody preparation was analyzed by immunohistochemistry (at a dilution of 1:5000) using tissues infected with a virus bearing the LacZ gene. Secondary antibodies were fluorescein-labeled affinity-purified goat anti-chicken IgY (1:2000 dilution, Aves Labs Cat.# F-1005).

Storage

Store at -20°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies at -80°C unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half its binding activity is lost.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.

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鸡的概念 在一次会议上，他与同事的一次谈话说服了西蒙博士再次尝试制造这种抗体，但是尝试把鸡作为宿主——鸡在进化上与包括人类在内的哺乳动物完全不同。结果表明:人蛋白抗原具有高度的免疫原性，抗体产量异常高，免疫试剂结合紧密，特异性高。 今天的Aves 从那时起，Aves Labs开创了在蛋白质中寻找高免疫原性肽序列的新算法，开发了针对鸡抗体的新的亲和纯化方法，并开发了许多其他有用的试剂，如BlokHen®阻断试剂和PrecipHen®免疫沉淀试剂。Aves实验室继续开发针对神经特异性抗原和表位标签的新型抗体试剂，以促进神经科学和分子生物学领域的科学进步。