Antibodies were prepared by injecting laying hens with purified recombinant human PRN protein. After a series of boosts, eggs were collected from hyperimmunized aniamsl and the IgY fraction prepared. Affinity purified antibodies were then isolated from the IgY fraction using a PRN protein column. Finally, affinity purified antibodies were mixed with the IgY fraction to make the final product. These antibodies have been validated with human, mouse and rat tissues.

Volume: 200 µL

Concentration: 10 mg/mL

Clonality: Polyclonal

Form: Mixture of IgY fraction (10 mg/mL) and affinity-purified antibodies (50 µg/mL)

Host Species: Chicken

Species Reactivity:Human, Mouse, Rat

Applications: ICC, IHC, WB

Protein Name / Synonyms: Major prion protein (PrP) (ASCR) (PrP27-30) (PrP33-35C) (CD antigen CD230)

Target Description: Prion protein is a ubiquitously-expressed, 27661 dalton protein (253 amino acids, NP_898902.1) of unclear function(s) in eukaryotes. This protein can exist in one of two conformational forms. One is its normally-folded form (PrPc or "cellular PrP"), which contains a mixture of alpha helical regions and a few beta-pleated sheets. The other is a misfolded form (PrPsc or "scrapie PrP") whose structure contains mostly beta-pleated sheets. This misfolded scrapie PrP form is cytotoxic, especially to nervous tissue, and can serve as a "seed," inducing adjacent, normally-folded PrP proteins to misfold into the cytotoxic conformation. In this sense, the scrapie PrP can be thought of as an infectious agent both within the organism as well between organisms.

Gene ID: PRNP ALTPRP PRIP PRP

Antibody Registry ID (RRID): AB_2313559

Physical State: Liquid

Production Notes: Chickens were immunized with a recombinant fragment of the human prion protein emulsified with Freund’s complete and incomplete adjuvants.After multiple injections, eggs were collected from the hens, and IgY fractions were prepared from the yolks.Affinity-purified antibodies were then prepared using an agarose column to which the recombinant protein fragments were covalently attached. Finally, affinity purified antibodies (50 µg/mL final concentration) were mixed with the IgY fraction (10 mg/mL final concentration) in a mixture containing glycerol (50% v/v) (to prevent freezing at -20°C) and 0.02% sodium azide.

Validation and Application Notes

IHC Dilution Range: 1:1000-1:2000

Quality Control

Antibodies were analyzed by immunocytochemistry (1:1000 dilution) using cells transfected with the prion protein cDNA.

Storage

Store at -20°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 2 years (provided they remain sterile). For longer storage periods, store at -80°C. Note that storage at this lower temperature will destroy some antibody activity due to water crystallization.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.

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鸡的概念 在一次会议上，他与同事的一次谈话说服了西蒙博士再次尝试制造这种抗体，但是尝试把鸡作为宿主——鸡在进化上与包括人类在内的哺乳动物完全不同。结果表明:人蛋白抗原具有高度的免疫原性，抗体产量异常高，免疫试剂结合紧密，特异性高。 今天的Aves 从那时起，Aves Labs开创了在蛋白质中寻找高免疫原性肽序列的新算法，开发了针对鸡抗体的新的亲和纯化方法，并开发了许多其他有用的试剂，如BlokHen®阻断试剂和PrecipHen®免疫沉淀试剂。Aves实验室继续开发针对神经特异性抗原和表位标签的新型抗体试剂，以促进神经科学和分子生物学领域的科学进步。