Volume: 500 µL

Concentration: 1 mg/mL

Clonality: Polyclonal

Form: Affinity Purified

Host Species: Goat

Species Reactivity: Chicken

Immunogen: IgY

Physical State: Liquid

Conjugate: FITC

Buffer: Phosphate-buffered saline (PBS) (pH 7.2), bovine serum albumin (0.2%, w/v), sodium azide (0.02%, w/v) (as an anti-microbial agent).

Specificity: Does not cross-react with mammalian IgGs

Production Notes: Goats were immunized with purified chicken IgY (whole protein) emulsified in Freund’s adjuvant. After multiple injections, goats were bled and serum collected. Antibodies were isolated using affinity chromatography and conjugated to fluorescein isothiocyanate.

Quality Control

Antibodies were analyzed using immunoelectrophoresis (see below) and immunocytochemistry. In this latter test, abundant fluorescence staining was observed in 3T3 cells after blocking with BlokHen® (1:10 dilution in PBS), incubation with chicken anti-vimentin antibodies (1:1000 dilution in PBS) and then incubation with GGHL-30F-2 (1:200 dilution in PBS).

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). For longer storageperiods, dilute the antibodies with sterile glycerol and store at -20°C. Do not freeze these antibodies unless you want to store them for longer periods of time.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.

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鸡的概念 在一次会议上，他与同事的一次谈话说服了西蒙博士再次尝试制造这种抗体，但是尝试把鸡作为宿主——鸡在进化上与包括人类在内的哺乳动物完全不同。结果表明:人蛋白抗原具有高度的免疫原性，抗体产量异常高，免疫试剂结合紧密，特异性高。 今天的Aves 从那时起，Aves Labs开创了在蛋白质中寻找高免疫原性肽序列的新算法，开发了针对鸡抗体的新的亲和纯化方法，并开发了许多其他有用的试剂，如BlokHen®阻断试剂和PrecipHen®免疫沉淀试剂。Aves实验室继续开发针对神经特异性抗原和表位标签的新型抗体试剂，以促进神经科学和分子生物学领域的科学进步。