H3K4me3MouseMonoclonalAntibody
Clonality:MouseIG1 | Source:Mouse | |
Applications:WB:1:500-1:2000 | Cross-Reactivity:HMRat | |
Concentration:100ug/100ul | Detectedband(bywestern)MW:15KD | |
Alt.Names
H3histoneantibody,HIST1H3Aantibody,Histonecluster1,H3aantibody
Specificity:
TheHistoneH3(trimethylK4)mousemonoclonalantibodycanrecognizeendogenousHistoneH3(trimethylK4)proteins.
Immunogen:
MousemonoclonalantibodyispreparedbyimmunizingsyntheticpeptidecoupledtoKLH.
StorageConditionandBuffer:
Storeat-20°C.Stableforoneyearfromthedateofshipment.1mg/mlinPBS,pH7.4,containing0.02%sodiumazideand50%glycerol
Westernblotanalysisof1)Hela,2)RatTestistissue,3)Raw264.7withHistoneH3(trimethylK4)MousemAbdilutedat1:1000.
H3K4methylationisoneofthegeneactivationMarkersandoneofthehottestepigenetictargetsinrecentstudies.H3K4me1,H3K4me2andH3K4me3haveacommonalityforgeneactivation,althoughtheirsubsetsplaydistinctrolesinmodulatingchromatinfunction(4).Amongwhich,H3K4me3occupiesasmanyas75%ofallhumangenepromotersinseveralcelltypes(e.g.,EScells),playingimportantroleinmammaliangeneexpression(1).H3K4me2decoratesgenomicregionsindependentlyofH3K4me3,althoughmostofitoverlapswithH3K4me3nearthetranscriptionstartsites(2).MonomethylatedH3K4(H3K4me1)alsoco-occupiesregionsnearthestartsiteswithH3K4me3.Apartfromthetranscriptionstartsite,H3K4me1,togetherwithH2K27acetylation,specifiesenhancerregions(3).
Inrecentstudies,H3K4hasbeendemonstratedtoberelatedstemcellself-renewalanddifferentiation(4).
H3K4methylationstatusescanbedetectedbyourH3K4methylationantibodyseries.
H3K4me3_monocolonal_Antibody.pdf
SDS_H3K4me3_Mouse_Monoclonal_Antibody.pdf
References:
- PanG,TianS,NieJ,ThomsonJA:Whole-genomeanalysisofhistoneH3lysine4andLysine27.CellStemCell2007,1:299-312.
- 2.BernsteinBE,KamalM,Lindblad-TohK,GingerasTR,etal:Genomicmapsandcomparativeanalysisofhistonemodificationsinhumanandmouse.Cell2005,120:169-181.
- 3.ViselA,BlowMJ,Liz,ZhangT,WrightC,ChenF,etal:CHIP-seqaccuratelypredictstissue-specificactivityofenhancers.Nature2009,457:854-858.
- 4.BingnanGuandMinGyuLee:HsitoneH3lysine4methyltransferasesanddemethylasesinself-renewalanddifferentiationofstemcells.Cell&Biosciences2013,3:39.
