Species: Human Host Species: Sf9 insect cells MW: 96 kDa Genbank #: NM_001098536 Tag(s): N-terminal FLAG-tag a.a: 2–858(end)Description:Human USP5 (Ubiquitin-Specific Protease 5) or Isopeptidase T (IsoT), GenBank Accession No. NM_001098536, a.a. 2-858(end) with an N-terminal FLAG-tag, MW=96 kDa, expressed in a baculovirus-infected Sf9 cell expression system. UniProt P45974 Synonym(s): USP 5, Ubiquitin-Specific Protease 5, Isopeptidase T, IsoT, USP-5Specific Activity: 14.3 pmole/min/µg Assay Conditions: Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and USP5 in a 50 µL reaction. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation = 340 nm, emission = 460 nm.Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 10% glycerol, 3 mM DTT, and 90 ?g/ml FLAG peptideFormat: Aqueous buffer solutionStorage / Stability:

>6 months at -80°C.

Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.Reference(s): 1. Dayal, S., et al. (2009). J. Biol. Chem. 284(8):5030-41.2. Reyes-Turcu, F.E., et al. (2008). J. Biol. Chem. 283(28):19581-92.Warning(s): Avoid freeze/thaw cyclesScientific Category: Ubiquitins: Deubiquitinase (DUB)Product Type: Recombinant ProteinData shown is lot-specific. Contact us for specific information on other lots.

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