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IsolationfromHEK293Cells:PARP1andTnks1
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●RNF146WWEAffinityResinSetTheTulipBiolabs,Inc.RNF146WWEAffinityResinSetisdesignedfortheisolationofpoly-ADP-ribosylatedproteinsfromculturedcellsandtissuesamples.ThroughtheuseofahighlyspecificWWEaffinityresin,poly-ADP-ribosylated(PARylated)proteinsareisolatedfromcellortissuelysateswithouttheuseofanti-PARantibodies. Theresinboundproteinscanbeelutedfromtheaffinityresin,andanalyzedbyimmunoblottingorothermethods.
Cat.#4306Setcontains1eachofthefollowing(canbeorderedseparately):
•WWEResin,0.5mgWWEfusionproteinsuppliedasaslurrycontaining ~50µLresin,Cat.#2334
•WWENegControlResin,0.5mgfusionproteinsuppliedasaslurrycontaining~50µLresin, Cat.#2335
TheWWEAffinityResin,Cat.#2334ishighlypurifiedGST-RNF146(100-175)fusionproteinconstructexpressedinE.coli,andboundtoglutathionebeads.
WWENegativeControlResin,Cat.#2335isidenticaltothe#2334resinexceptforR163Asubstitution,whicheffectivelyabolishesPARbinding. Thenegativecontrolresinisusefultocontrolfornon-specificbinding,anditsuseisoptional.
Pleaseseethe Cat.#4306 Datasheet(pdf) foracompletedescription.