Description
TheCultreCoat®VascularPermeABIlityAssayemploysasimplifiedBoydenchamberdesignwithaonemicronpolyethyleneterephthalate(PET)membranecoatedwithcollagenI.Portswithinthemigrationchamber(top)allowaccesstotheassaychamber(bottom)withoutdismantlingthedevice.Thisdesigniseasiertouse,preventscontamination,andisadaptableforrobotichighthroughputsystems.Theassaychambermaybedirectlyanalyzedinaplatereader,eliminatingtransferstepsthatintroduceadditionalvariabilitytotheassay.
Theconfluentcellmonolayerformsabarrierthroughcell-celladhesionmechanismssuchasjunctions(adherens,tight,andgap)anddesmosomes,andthisbarrierrestrictsFITC-Dextrantotheinserts.Disruptionofthesecell-celladhesionmechanismscreatesgapsintheintercellularspacethatallowdiffusionofFITC-Dextranintothebottomchamber,sotheamountofFITC-Dextranthatdiffusesisrelatedtotheamountofintercellularspace,whichisrepresentativeofvascularpermeability.
| Catalog#3475-096-Kincludes: | ||
| CatalogNumber | Description | Qty |
| 3475-096-01 | CultreCoat®96WellVascularPermeabilityChamber | 1 |
| 3475-096-02 | CultreCoat®FITC-Dextran | 1 |
| Catalog#3475-024-Kincludes: | ||
| CatalogNumber | Description | Qty |
| 3475-024-01 | CultreCoat®24WellVascularPermeabilityChamber | 1 |
| 3475-096-02 | CultreCoat®FITC-Dextran | 1 |
