Description
Cultrex®EmbryoidBodyFormationKitincludesROCKInhibitorY-27632,whichsignificantlyincreasessurvivalofPSCsinsinglecellculture,aspecializedEBFormationMatrixtostimulatecellaggregationanddevelopmentofEBs,anda96-wellEBFormationPlate,thatallowsforcontroloftheEBsizeandshape.Notransferstepisrequiredfordifferentiation.
Cultrex®EmbryoidFormationMatrixisapurifiedbasementmembraneproteinfromEngelbreth-Holm-Swarm(EHS)tumor.Cultrex®EmbryoidFormationMatrixhasbeendeveloped,producedandqualifiedtobeusedwithCultrex®EmbryoidFormationKit.
Features:
- HighEBgrowthsuccessrate
- UniformandconsistentsizedEBs
- Formdirectlyinplate,notransfersteprequired
- High-throughputformat
- ROCKInhibitorY-27632increasesPSCsurvivalrate
- SpecializedEBFormationMatrixstimulatescellaggregation
Qualitycontrolspecifications:
- Mousecoloniesareroutinelyscreenedforpathogensusing mouseantibodyproduction(MAP)testing.
- Engelbreth-Holm-Swarm(EHS)tumorisLDEV-free.
- Nobacterialorfungalgrowthdetectedafterincubationat37°Cfor14daysfollowingUSPsterilitytestingguidelines.
- Endotoxinconcentration≤20EU/mlbyLimulusAmoebocyteLysate(LAL)assay.
- NomycoplasmacontaminationdetectedbyPCR.
- BIOLOGicalactivitydeterminedforeachlot:
- EmbryoidBodyFormationAssay.
| Catalog#3550-096-Kincludes: | ||
| CatalogNumber | Description | Qty |
| 3550-096-01 | Cultrex®10XEBFormationMatrix | 1 |
| 3550-096-02 | 500XROCKinhibitorY-27632 | 1 |
| 3550-096-03 | EBFormationPlate | 1 |


