Description
TACS®2TdTCorekitutilizeTrevigen’suniquecationoptimizationsystemtoenhancelabelingwithinparticulartissues.Thekitalsofeatures aproprietarylabelingbufferthatcontainsnotoxiccomponents(e.g.sodiumcacodylate).AhighlypurifiedformoftheTdTenzymeisincludedfortheenzymaticincorporationofbiotinylatednucleotides.Biotinlabelingisdetectedusingstreptavidin-horserADIshperoxidase,andtheresearcher’schoiceofcolorimetricsubstrates orafluorescentconjugateofstreptavidinmaybeusedforvisualization byepifluorescencemicroscopy.
FEATURES:
- TdTbasedinsitulabeling.
- Cationoptimizationforhighsignalandlowbackground.
- IncludesexclusiveCytonin™permeABIlizationreagent.
- IncludesTACS-Nuclease™controlreagents.
- LightmicroscopyorFluorescencemicroscopyoptions.
APPLICATIONS:
- InsitudetectionofapoptosisbyTUNELinfixedfrozen,paraffinembedded,orplasticembeddedcellsandtissues.
| Catalog#4810-30-CKincludes: | ||
| CatalogNumber | Description | Qty |
| 4800-30-06 | Streptavidin-HRP | 1 |
| 4810-30-02 | TACS®2TdTLabelingBuffer | 1 |
| 4810-30-03 | TACS®2TdTStopBuffer | 1 |
| 4810-30-04 | TACS®2TdTdNTP | 1 |
| 4810-30-05 | TdTEnzyme | 1 |
| 4810-30-09 | 50xCobaltCation | 1 |
| 4810-30-10 | 50xMagnesiumCation | 1 |
| 4810-30-14 | 50xManganeseCation | 1 |
AutophagyinPancreaticAcinarCellsinCaerulein-treatedMice:ImmunolocalizationofRelatedProteinsandTheirPotentialasMarkersofPancreatitis
Zhang,L.,Zhang,J.,Shea,L.,Xu,L.,Tobin,G.,Knapton,A.,Sharron,S.,andRouse,R.
ToxicolPathol,Feb2014;42:435–457.
http://tpx.sagepub.com/cgi/content/abstract/42/2/435
Smad7inducesplasticityintumor-infiltratingTh17cellsandenablesTNF-alpha-mediatedkillingofcolorectalcancercells
Rizzo,A.,DeMare,V.,Rocchi,C.,Stolfi,C.,Colantoni,A.,Neurath,M.F.,Macdonald,T.T.,Pallone,F.,Monteleone,G.,Fantini,M.C.,
Carcinogenesis,Feb2014;10.1093/carcin/bgu027.
http://carcin.oxfordjournals.org/cgi/content/abstract/bgu027v2
Differentialeffectsof2-and3-seriesE-prostaglandinsoninvitroexpansionofLgr5+colonicstemcells
Fan,Y.Y.,Davidson,L.A.,Callaway,E.S.,Goldsby,J.S.andChapkin,R.S.
Carcinogenesis,Mar2014;35:606-612
http://carcin.oxfordjournals.org/cgi/content/abstract/35/3/606
