Description
3DCulturesexhibitcellularbehaviorsandmorphologiessimilartothoseseeninvivo;however,theadaptationofthesemodelsforstudyingbiochemicalprocesseshasbeenimpededbythechallengeofseparatingintactcellsfromextracellularmatrixhydrogels. Commonly,proteasesareemployedtodegradetheseextracellularproteins;however,proteasesalsodegradeproteinsonthecellsurfaceandmaycarryoverintolysatepreparations.Non-enzymaticmethodologieshavealsobeendescribedfordepolymerizing extracellularmatrixproteins,althoughtheimplementationoftheseprotocolsremainsproblematicforsomeresearchers.TheCultrex®3DCultureCellHarvestingKitdepolymerizesBMEandLamininIhydrogels,non-enzymatically,providinganoptimizedandstandardizedsolutionfortheisolationof3Dstructuresandpreparationofcelllysatesforsubsequentbiochemicalanalysis.
| CatalogNumber | Description | Qty |
|---|---|---|
| 3448-020-01 | 3-DCulture10XCellHarvestingBuffer | 1 |
| 3448-020-02 | 3-DCulture10XCellWashBuffer | 1 |
| 3448-020-03 | 3-DCultureSampleBuffer | 1 |
| 3448-020-04 | 3-DCulture10XLoADIngBuffer | 1 |
| 2275-PC-020 | HumanAnti-G3PDHAntibody | 1 |
Trevigen公司位于美国马里兰州的高科技走廊，是一家有12年历史、成长迅速的生物技术公司，其产品和技术的发展方向主要在癌症研究领域，重点强调细胞凋亡、DNA损伤和修复，以及癌症细胞功能和行为。Trevigen品牌的分销商遍及北美，欧洲和环太平洋地区。关于DNA的损伤分析技术的研究，Trevigen获得美国国立卫生研究院（NIH）的SBIR项目资助。
