Description

Cultrex®3DCultureMatrixLamininI ispurifiedfromEngelbreth-Holm-Swarm(EHS)tumorand isprovidedatahighconcentrationthatiscapableofpolymerizingtoforma hydrogelat37°C.LamininIisamajorcomponentofthebasementmembranewhich isacontinuoussheetofspecializedextracellularmatrixthatformsaninterfacebetweenendothelial,epithelial,muscle,orneuronalcellsandtheiradjacentstromaandthatplaysanessentialroleintissueorganizationbyinfluencingcelladhesion,migration,proliferation,anddifferentiation.

Cultrex®3DCultureMatrixLamininI isapurifiedbasementmembraneprotein thathasbeendeveloped,producedandqualifiedspecificallyforusein3Dculturestudies.

Qualitycontrolspecifications:

• Mousecoloniesareroutinelyscreenedforpathogensusing mouseantibodyproduction(MAP)testing.
• Engelbreth-Holm-Swarm(EHS)tumorisLDEV-free.
• Nobacterialorfungalgrowthdetectedafterincubationat37°Cfor14daysfollowingUSPsterilitytestingguidelines.
• Endotoxinconcentration≤20EU/mlbyLimulusAmoebocyteLysate(LAL)assay.
• Proteinconcentrationof6mg/ml.
• NomycoplasmacontaminationdetectedbyPCR.
• ProvidedinDulbecco’sModifiedEagle’smedium(DMEM)withoutphenolred,with10µg/mlgentamicinsulfate.
• Purity ≥90%(SDS-PAGE).
• GelstABIlitytestedforaperiodof14daysat37°C.
• BIOLOGicalactivitydeterminedforeachlot:
  • 3-DCulture:LamininIpromotesdifferentiationofahumanepithelialcelllinederivedfrommammarygland(MCF-10A)intoacinarstructures.
  • CellAttachment:TestedfortheabilitytopromotecellattachmentandspreADIngofMG63humanosteosarcomacells.

Trevigen公司位于美国马里兰州的高科技走廊，是一家有12年历史、成长迅速的生物技术公司，其产品和技术的发展方向主要在癌症研究领域，重点强调细胞凋亡、DNA损伤和修复，以及癌症细胞功能和行为。Trevigen品牌的分销商遍及北美，欧洲和环太平洋地区。关于DNA的损伤分析技术的研究，Trevigen获得美国国立卫生研究院（NIH）的SBIR项目资助。