Product Highlights:
TruSeq Small RNA Library Preparation Kits provide reagents to generate small RNA libraries directly from total RNA. MicroRNAs (miRNAs) generated by Dicer processing are efficiently targeted by the included modified adapters.
These kits enable multiplexed sequencing with the introduction of 48 unique indexes, allowing miRNA and small RNA discovery and profiling throughput to match the unparalleled output of Illumina sequencing. Indexes are added in a universal amplification reaction, greatly reducing ligation bias and ensuring accurate measurement of miRNA expression.
Workflow improvements enable streamlined sample preparation, allowing economical studies covering all small RNA transcripts in any species. Compatible applications include finding novel miRNAs, characterizing variation such as isomers with single-base resolution, and analyzing differential expression without prior assumptions.
TruSeq Small RNA Sample Datasets
The Human Brain Reference RNA (HBRR) and Universal Human Reference RNA (UHRR) samples were prepared using the TruSeq Small RNA Library Prep Kit. These libraries were sequenced on the MiniSeq System using the MiniSeq High Output Reagent Kit at a 1 x 36 bp read length configuration. The total yield was 0.91 Gb with 97% of bases at or above Q30.
Browse the Data In BaseSpace Sequence Hub:
- View Run (primary analysis and metrics)
- View Project (secondary analysis with BaseSpace Apps)
Specifications:
| Assay Time | ~10.5 hours |
| Hands-On Time | ~30 minutes |
| Input Quantity | 25 to 100 ng total RNA from species with polyA tails |
| Content Specifications | Captures the coding transcriptome with strand information |
| Mechanism of Action | Oligo-dT beads capture polyA tails |
| Multiplexing | Up to 24-plex pooling with additional NeoPrep runs |
| Sample Throughput | Up to 16 samples per NeoPrep library card |
| Species Category | Drosophila,Any Species,Mouse,Zebrafish,Human,Rat,Plant,Nematode |
| System Compatibility | MiSeq,NextSeq 550,MiniSeq,NextSeq 500 |
| Specialized Sample Types | FFPE,Low Input |
| Technology | Sequencing |
| Automation Capability | Liquid Handling Robots |
| Variant Class | Novel Transcripts,Transcript Variants |
| Method | miRNA and Small RNA Sequencing |
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