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商品描述
Specifications
SPECIESREACTIVITY: | Human |
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TESTEDAPPLICATIONS: | ELISA,WB |
APPLICATIONS: | WesternBlot:1:500-1:1,000 IHC(P):1:500-1:1,000 ELISA:Proposedilution1:10,000. Determiningoptimalworkingdilutionsbytitrationtest. |
USERNOTE: | Optimaldilutionsforeachapplicationtobedeterminedbytheresearcher. |
POSITIVECONTROL: | 1)Cat.No.1202-A431CellLysate |
IMMUNOGEN: | Ni-NTApurifiedtruncatedrecombinant4E-BP1expressedinE.ColistrainBL21(DE3). |
HOSTSPECIES: | Mouse |
Properties
BUFFER: | Asciticfluidcontaining0.03%sodiumazide. |
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STORAGECONDITIONS: | 4E-BP1monoclonalantibodycanbestoredat-20˚C,stableforoneyear.Aswithallantibodiescareshouldbetakentoavoidrepeatedfreezethawcycles.Antibodiesshouldnotbeexposedtoprolongedhightemperatures. |
CLONALITY: | Monoclonal |
ISOTYPE: | IgG1 |
CONJUGATE: | Unconjugated |
AdditionalInfo
ALTERNATENAMES: | Phosphorylatedheat-andacid-stableproteinregulatedbyinsulin1,4E-BP1,BP-1,4EBP1,PHAS-I |
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ACCESSIONNO.: | Q13541 |
PROTEINGINO.: | 34921508 |
OFFICIALSYMBOL: | EIF4EBP1 |
GENEID: | 1978 |
Background
BACKGROUND: | 4E-BP1(eukaryotictranslationInitiationFactor4EBindingProtein1),alsocalledELF4EBP1/BP-1/PHAS-I,whichislocatedonchromosome8p12,with118-aminoacidprotein(about13kDa).BindingofeIF4EBP1toeIF4EisreversIBLeandisdependentonthephosphorylationstatusofeIF4EBP1.NonphosphorylatedeIF4EBP1willbindstronglytoeIF4Ewhile(24kDa),thephosphorylatedformwillnot.Akt,TOR,MAPkinase,S6kinase,andCdc2areknownkinasescapableofinactivatingeIF4EBP1bindingtoeIF4Ebyphosphorylatingeitherthreonines35,45,69orserine64.Although,notallphosphorylationeventsequallyblocktheeIF4EBP1-eIF4Einteraction. |
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REFERENCES: | 1)Pause,A.etal.1994.Nature.371:762–767. | 2)Fadden,P.etal.1997.J.Biol.Chem.272:10240–10247. |