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Prosci/BDNF Recombinant Protein/40-496/0.01 mg

价格
¥4500.00
货号:40-496
浏览量:70
品牌:ProSci
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商品描述

Specifications

SPECIES:Human, Mouse, Rat
SOURCE SPECIES:E. coli
SEQUENCE:MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR
TESTED APPLICATIONS:
BIOLOGICAL ACTIVITY: Biological activity was determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 for this effect is 0.5 - 1.0 μg/mL.

Properties

PURITY:Greater than 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin level is less than 0.1 ng per μg (1EU/μg).
PHYSICAL STATE:Lyophilized
STORAGE CONDITIONS:The lyophilized BDNF recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted BDNF is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing BDNF recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Additional Info

ALTERNATE NAMES:ANON2, BULN2, Brain-derived neurotrophic factor, Abrineurin, BDNF
ACCESSION NO.:NP_001137277.1
PROTEIN GI NO.:219842288

Background

BDNF is a member of the NGF family of neurotrophic growth factors. Like other members of this family, BDNF supports neuron proliferation and survival. BDNF can bind to a low affinity cell surface receptor called LNGFR, which also binds other neurotrophins such as NGF, NT-3 and NT-4. However, BDNF mediates its neurotrophic properties by signaling through a high affinity cell surface receptor called gp145/trkB. BDNF is expressed as the C-terminal portion of a 247 amino acid polypeptide precursor, which also contains a signal sequence of 18 amino acid residue and a propeptide of 110 amino acid residues. Recombinant human BDNF is a 27.0 kDa homodimer of two 119 amino acid subunits linked by strong non-covalent interactions. Human and Mouse BDNF sequences are identical.
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