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商品描述
Specifications
SPECIES: | Human |
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SOURCESPECIES: | HEK293cells |
SEQUENCE: | Arg29-Asp513 |
FUSIONTAG: | HisTag |
TESTEDAPPLICATIONS: | WB |
APPLICATIONS: | ThisrecombinantproteincanbeusedforWB.Forresearchuseonly. |
Properties
PURITY: | >95%asdeterminedbySDS-PAGE. |
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PREDICTEDMOLECULARWEIGHT: | 55.2kDa(Proprotein),34.5kDa(MatureForm)and20.1kDa(propeptide) |
PHYSICALSTATE: | Lyopholized |
BUFFER: | 50mMtris,100mMNaCl,pH7.5 |
STORAGECONDITIONS: | LyophilizedProteinshouldbestoredat-20˚Corlowerforlongtermstorage.Uponreconstitution,workingaliquotsshouldbestoredat-20˚Cor-70˚C.Avoidrepeatedfreeze-thawcycles. |
AdditionalInfo
ALTERNATENAMES: | ADAM12,MCMP,MLTN,MLTNA,MCMPMltna,MeltrinAlpha |
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ACCESSIONNO.: | AAH60804.1 |
Background
ADisintegrinAndMetalloprotease12(ADAM12)isalsoknownasMeltrin-alphaandMCMP,isamemberoftheADAMandMDC(Metalloprotease,Disintegrin,Cysteine-rich)proteinfamily.Membersofthisfamilyaremembrane-anchoredproteinsstructurallyrelatedtosnakevenomdisintegrins,andhavebeenimplicatedinavarietyofBIOLOGicalprocessesinvolvingindiverseprocessessuchasasthma,development,angiogenesisandcancerthroughtheiractivitiesincelladhesion/fusion,membraneproteinshedding,andsignaltransduction.Over30membershavebeenidentifiedandabouthalfofthemareactivemetalloproteasessuchasADAM8,9,10,12and17/TACE.ADAM12hasfourIsoform,Isoform1isexpressedinplacentaandskeletal,cardiac,andsmoothmuscle.Isoform2seemstobeexpressedonlyinplacentaorinembryoandfetus.Bothformswereexpressedinsometumorcellslines.ADAM12Involvedinskeletalmuscleregeneration,specificallyattheonsetofcellfusionandalsoinvolvedinmacrophage-derivedgiantcells(MGC)andosteoclastformationfrommononuclearprecursors. |