BIOMYC-3 is based on the antibiotic ciprofloxacin, which is a member of the fluoroquinolone group and inhibits synthesis of the enzyme DNA gyrase which is responsible for supercoiling in bacterial DNA. Many mycoplasma species have been found to be sensitive to BIOMYC-3, including A. laidlawii, M. orale, M. hyorhinis, M. fermentans, and M. arginini. These species are responsible for most mycoplasma contamination in cell culture. At the concentrations recommended for use, no cytotoxic effects have been found so far, and the treatment is quite easy to perform.The treatment consists of adding the antibiotic to the growth medium for 12 days, and changing the medium every three days. On completion of the treatment, the cells should be grown on media without antibiotics for at least two weeks and then re-tested for the presence of mycoplasma. If the culture tests positive, the course of treatment should be repeated. Treatment with ciprofloxacin does not damage or alter the cells. This antibiotic should not be used repeatedly in cell cultures; otherwise, the mycoplasmas are liable to develop resistance to it.
PromoKine细胞生物学(Cell Biology)是在显微、亚显微和分子水平三个层次上,研究细胞的结构、功能和各种生命规律的一门科学。细胞生物学由细胞学发展而来,细胞学是关于细胞结构与功能(特别是染色体)的研究。现代细胞生物学从显微水平、超微水平和分子水平等不同层次研究细胞的结构、功能及生命活动。在我国基础学科发展规划中,细胞生物学与分子生物学、神经生物学和生态学并列为生命科学的四大基础学科。