Species: Human Host Species: Sf9 insect cells MW: 87 kDa Genbank #: AF035121 Tag(s): N-terminal GST-tag a.a: 805–1356 Description:Human VEGFR2 (GenBank Accession No. AF035121), a.a. 805-1356 with N-terminal GST-tag, MW=87 kDa, expressed in a Baculovirus infected Sf9 cell expression system. Synonym(s): KDR, Kinase Insert Domain Receptor, VEGFR2, Vascular Endothelial Growth Factor Receptor 2, CD309, FLK-1, Fetal Liver Kinase 1 Specific Activity: ≥400pmol/min/μg Unit Definition: One unit of KDR activity is defined as the amount of enzyme required to produce 1 pmol of phosphorylated Tyr peptide 1 per min at 37°C. Assay Conditions: Assay was done in Kinase buffer containing 0.2 mM DTT using Poly- (Glu4:Tyr)-biotin substrate (0.2 mg/ml) and 20 mM ATP. Reaction was done at 30°C for 40 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega). Formulation: 40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween, 20% glycerol, 3 mM DTT Format: Aqueous buffer solution Storage / Stability: At least 6 months at –80°C. Application(s): Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Reference(s): 1. Meyer, M. et al., EMBO J. 18, 363-374 (1999). 2. Rahimi, N. et al., J. Biol Chem 275, 16986- 16992 (2000). Warning(s): Avoid freeze/thaw cycles Scientific Category: Kinase/Receptor Tyrosine Product Type: Recombinant Protein Data shown is lot-specific. Contact us for specific information on other lots.

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