• 货号：	AS12 2115
  抗体名：	兔抗拟南芥细胞质核糖体蛋白L37多克隆抗体
  抗体英文名：	RPL37|ribosomal protein L37,cytoplasmic
  浓度：	见说明书
  应用范围：	western blot (WB)
  宿主：	兔
  适应物种：	植物
  标记物：	无
  克隆性：	否
  保存条件：	低温
  亚型：	IgG
  规格：	50 µl

  供应商：上海经科化学科技有限公司 

   

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  RPL37|ribosomal protein L37,cytoplasmic介绍：

  货   号：AS12 2115

  中文名称：兔抗拟南芥细胞质核糖体蛋白L37多克隆抗体

  英文名称：RPL37|ribosomal protein L37,cytoplasmic

  应用：western blot (WB)

  规格：50 µl

  价格：5175元

   

  RPL37|ribosomal protein L37,cytoplasmic简介：

  PRODUCT INFORMATION background   RPL37 (Ribosomal protein L37) belongs to the ribosomal protein L37e family (cytosolic large ribosomal subunit) and binds to the 23S rRNA. immunogen   recombinant, full length RPL37 of Chlamydomonas reinhardtii, A8IBG1, expressed in E.coli host   rabbit clonality   polyclonal purity   serum format   lyophilized quantity   50 µl reconstitution   For reconstitution add 50 µl of sterile water. storage   store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes. tested applications   western blot (WB) related products   AS13 2650 | anti-L13-1 | 60S ribosomal protein L13-1, rabbit antibodies AS09 478| anti-L13-1 | 60S ribosomal protein L13-1, rabbit antibodies AS08 331| anti-L-30 | 50S ribosomal protein L30, rabbit antibodiesAS11 1738| anti-Rpl1 | 50S ribosomal protein L1, rabbit antibodies AS15 2876 | anti-RPL2 | ribosomal protein L2 (chloroplastic), rabbit antibodies AS14 2780 | anti-RPL33 | 50S ribosomal protein L33, chloroplastic, rabbit antibodies AS14 2781 | anti-RPL36 | 50S ribosomal protein L36, chloroplastic, rabbit antibodiesAS15 2875 | anti-RPS1 | ribosomal protein S1 (chloroplastic), rabbit antibodiesAS12 2114 | anti-RPS12 | ribosomal protein S12, chloroplastic, rabbit antibodiesAS14 2779 | anti-RPS15 | 30S ribosomal protein S15, chloroplastic, rabbit antibodiesAS10 719 | anti-Rps2 | anti-ribosomal subunit 2 (cytoplasmic), rabbit antibodiesAS08 309 | anti-S1 | anti-30S ribosomal protein S1, rabbit antibodiesAS12 2111 | anti-S14 | 40S ribosomal protein S14-1 , rabbit antibodiesAS15 3068 | anti-S4 |   mitochondrial ribosomal small subunit protein S4, rabbit antibodiesAS15 3067 | anti-S10 |  mitochondrial ribosomal small subunit protein S10, rabbit antibodies​AS15 3069 | anti-L16   mitochondrial ribosomal large subunit protein L16, rabbit antibodies additional information   to be added when available

  APPLICATION INFORMATION
  recommended dilution		1 : 10 000 with standard ECL (WB)
  expected | apparent MW		10.5 kDa
  confirmed reactivity		Arabidopsis thaliana, Chlamydomonas reinhardtii
  predicted reactivity		Glycine max, Solanum lycopersicum, Oryza sativa, Pinus sp., Ricinus communis,  Micromonas sp., Ostreococcus lucimarinus, Volvox carteri
  not reactive in		no confirmed exceptions from predicted reactivity are currently known
  additional information		to be added when available
  selected references		Ramundo et al. (2013). Repression of Essential Chloroplast Genes Reveals New Signaling Pathways and Regulatory Feedback Loops in Chlamydomonas. The Plant Cell.

   

  application example 

   

  10 µg of total protein from Chlamydomonas reinhardtii extracted with standard lysis buffer (Tris-HCl pH 6.8 50mM, 10mM EDTA, 2% SDS, Sigma protease inhibitors)  were separated on 15 % SDS-PAGE and blotted to nitrocellulose membrane using a wet transfer cell. Blot was blocked in TBS-T containing 5% non-fat dry milk  for 1h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1: 10 000 in TBS-T containing 5% non-fat dry milk  for 1h at RT with agitation. The antibody solution was decanted and the blot was washed  3 times for 15 min  in TBS-T containing 1% non-fat dry milk with agitation. Blot was incubated in secondary antibody (anti-rabbit IgG horse radish peroxidase conjugated, from Promega) diluted to 1:0 000 in TBS-T containing 1% non-fat dry milk  for 1h at RT with agitation. The blot was washed as above  and developed for 1 min with ECL according to the manufacturers instructions. Exposure time was 30 seconds.

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