Antigen/Purification: Collapse
The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser43 of mouse troponin I, cardiac (cTnI).
The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
Biological Significance: Collapse
Troponin I (TnI) is 1 of 3 subunits, along with troponin C (TnC) and Troponin T (TnT) of troponin complex found in cardiac (cTnI) and fast skeletal (fsTnI) muscle (Noland et al, 1995). Protein kinase C-mediated phosphorylation of cardiac myofilament proteins has been shown to depress the actomyosin interaction and may be important during heart failure. (Montgomery et al, 2002) Studies indicate that phosphorylation of Ser43 and Ser45 of cardiac troponin I plays a substantial role in the PKCmediated depression. (Montgomery et al, 2002).
Storage
100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.
Product Specific References
Thoemmes, S. F., Stutzke, C. A., Du, Y., Browning, M. D., Buttrick, P. M., & Walker, L. A. (2014). Characterization and validation of new tools for measuring site-specific cardiac troponin I phosphorylation. Journal of immunological methods, 403(1), 66-71. PMID: 24291343
Walker, L. A., Fullerton, D. A., & Buttrick, P. M. (2013). Contractile protein phosphorylation predicts human heart disease phenotypes. American Journal of Physiology-Heart and Circulatory Physiology, 304(12), H1644-H1650. PMID: 23564307
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