Antigen/Purification: Collapse
The antigen is a purified guinea pig calretinin.
The antibody is protein A purified sheep serum.
Biological Significance: Collapse
Calretinin is a calcium-binding protein involved in calcium signaling. It is abundantly expressed throughout the central nervous system, retina and many other tissues. Studies have shown that calretinin may play a role in neuroprotection by buffering intracellular calcium (Lukas and Jones 1994, Weisenhorn et al., 1996). Additionally, calretinin selectively stains mesothelial cells thus making it an important marker for differentiating malignant mesothelioma from carcinomas (Attanoos et al., 2000).
Storage
100 µl in 10 mM Tris, 100 mM NaCl.
For long term storage –20° C is recommended in undiluted aliquots. Stable at –20° C for at least 1 year. Avoid freeze/thaw cycles.
General References
Attanoos RL, Dojcinov SD, Webb R, Gibbs AR. (2000) Anti-mesothelial markers in sarcomatoid mesothelioma and other spindle cell neoplasms. Histopathology.37(3):224-31.
Isaacs KR, Winsky L, Strauss KI, Jacobowitz DM. (1995) Quadruple colocalization of calretinin, calcitonin gene-related peptide, vasoactive intestinal peptide, and substance P in fibers within the villi of the rat intestine. Cell Tissue Res., 280(3):639-51.
Lukas W, Jones KA (1994) Cortical neurons containing calretinin are selectively resistant to calcium overload and excitotoxicity in vitro. Neurosci. 61(2):307-16.
Weisenhorn DMV, Weruaga-Prieto E, Celio MR (1996) Calretinin immunoreactivity in organotypic cultures of the rat cerebral cortex: effects of serum deprivation. Exp. Brain Res. 108(1):101-112.
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