Antigen/Purification: Collapse
The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho Ser153 of human Hsp70.
The antibody is prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
Biological Significance: Collapse
The Hsp70 family of heat shock proteins are considered stress-induced survival proteins as they are expressed when exposed to factors such as heat, hypoxia, oxidative stress, altered pH or by underlying factors like cancer (Daugaard et al. 2007). Hsp70 proteins are able to bind hydrophobic residues on misfolded proteins, thereby preventing their aggregation and thus serving important roles in protein homeostasis. To date, there have been 8 members of the family identified; the majority of which are found in the cytosol though some have specific function in tissue or an organelle (Murphy, 2013). Hsp70 has also been shown to play a major role in cancer; from tumor grade to prognosis, as well as chemotherapeutic drug resistance (Ciocca et al, 2005). There have been several phospho-serine and threonine sites identified within Hsp70, the role of each one has yet to be determined.
Storage
100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
For long term storage –20° C is recommended. Stable at –20° C for at least 1 year.
General References
Daugaard M, Rohde M, Jäättelä M (2007) The Heat Shock Protein 70 Family: Highly homologous proteins with overlapping and distinct functions. FEBS Lett, 581: 3702-10.
Ciocca DR, Calderwood SK (2005) Heat Shock Proteins in Cancer: Diagnostic, Prognostic, Predictive, and Treatment Implications. Cell Stress Chaperones, 10: 86-103.
Murphy ME (2013) The Hsp70 Family and Cancer. Carcinogenesis, 34:1181-8.
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