More Information Product Details Synonyms Product Type Properties Source/Host Sequence Crossreactivity Specificity Biological Activity MW Purity Endotoxin Content Concentration Reconstitution Formulation Other Product Data Shipping and Handling Shipping Short Term Storage Long Term Storage Handling Advice Use/Stability Documents MSDS Product Specification Sheet Datasheet

NTN1

Protein

HEK 293 cells

Human netrin-1 (aa 25-604) is fused at the C-terminus to the Fc portion of human IgG1.

HumanMouseRat

Binds to human, mouse and rat UNC5B.

Induces pluripotent stem cell (iPS) formation and improves reprogramming efficiency of human and mouse somatic cells by inhibiting apoptosis mediated by the receptors DCC or UNC5B (at 150ng /ml). Induces axon outgrowth.

~90kDa (SDS-PAGE)

≥95% (SDS-PAGE)

0.1mg/ml after reconstitution.

Reconstitute with 100μl sterile distilled water.

Lyophilized. Contains PBS plus protein stabilizer. No BSA included. The protein stabilizer shows no interference with protein activity.

UniProt link O95631: Netrin-1 (human)

BLUE ICE

+4°C

-20°C

After reconstitution, prepare aliquots and store at -20°C.Avoid freeze/thaw cycles.PBS containing at least 0.1% BSA should be used for further dilutions.

Stable for at least 6 months after receipt when stored at -20°C.

Download PDF

Download PDF

Netrin-1 controls guidance of CNS commissural axons and peripheral motor axons. Its association with either DCC or some UNC5 receptors will lead to axon attraction or repulsion, respectively. It also serve as a survival factor via its association with its receptors which prevent the initiation of apoptosis. Netrin-1 is also Involved in tumorigenesis by regulating apoptosis. Netrin-1 promotes atherosclerosis by retaining macrophages in the artery wall. Netrin-1 also governs induced pluripotent stem cell (iPS) formation and improves reprogramming efficiency of human and mouse somatic cells by limiting apoptosis mediated by Netrin-1 receptors DCC or UNC5b.Product References

DCC constrains tumour progression via its dependence receptor activity: M. Castets, et al.; Nature 482, 534 (2011)

The amyloid precursor protein regulates netrin-1 mediated commissural axon outgrowth: N. Rama, et al.; J. Biol. Chem. 287, 30014 (2012)

Netrin-1 regulates somatic cell reprogramming and pluripotency maintenance: D. Ozmadenci, et al.; Nat. Commun. 6, ID7398 (2015)

>>> 更多资讯详情请访问蚂蚁淘商城