产品详细
Norgen Biotek是来自加拿大的著名生物技术公司,生产基于技术上的核酸和蛋白质纯化及富集产品。Norgen Biotek 在2003年获得加拿大国家研究委员会颁发的科技创新奖。目前该公司的产品多达40余种,覆盖基因组和蛋白组,广泛地应用于不同的领域。
提纯RNA的方法一般有两种,离心法和有机抽取法(例如Trizol)。Norgen Biotek是世界上*家真正意义上的生产以离心柱(Spin Columns)为平台,并能纯化所有种类RNA(包括小分子RNA)的公司。
Norgen Biotek和其他公司生产的以离心纯化为原理的试剂盒比:
- 其他公司一般都使用硅材料(silica matrix)为填充物。硅材料的缺点是在没有酚存在的情况下,不能回收所有的RNA,使得其纯化效率很低,特别是对小RNA(例如microRNA),其回收率几乎为零。这也是为什么很多客户在使用了其他公司的以离心为原理的总RNA纯化产品后,往往得不到预想结果的原因。
- 即使对少量细胞的总RNA提纯,也具有很高的线性(linearity on low numbers of cells)。在细胞数量多的情况下,其他公司的产品表现还可以,但是在细胞数量少的情况下,其纯化效率会大大降低,为提高效率往往需要通过其他的途径,例如Carrier RNA。Norgen的试剂盒可以从一个细胞中提纯出高质量的总RNA,在同行业中处于地位。
- Norgen的试剂盒完全满足所有的下游实验应用。
Norgen Biotek和其他公司生产的以Trizol原理的试剂盒比较:
- 在这些方法中,层析会造成有机试剂的残留,从而抑制许多下游实验的进行。
- 许多实验室由于健康和环境的因素,已经放弃了以酚抽为原理的提纯方法。
- 对大样品,使用Trizol的效果还可以,但同时也由于基因组DNA的增加,会阻止RNA的沉淀,从而间接地降低了纯化效率
- 对小量样品,使用Trizol的效果往往不佳。
- 使用Trizol中,乙醇沉淀(ethanol precipitation)效果的好坏和RNA的量有密切的关系,因此对于少量样品,沉淀法往往得不到zui终的RNA产物。
- 由于许多实验(e.g. qRT-PCR),并不需要很多量的RNA,因此Trizol方法已经逐渐被其他方法所代替。
Norgen Biotek采用的柱子填充物是一种新型的,并有保护的非硅胶材料。可以在没有酚等有害有机溶剂存在的情况下,纯化包括microRNA在内的所有RNA,而且其纯化回收效率高达99%。人们一直认为如果要纯化microRNA,必须要用Trizol,而不是离心法。这种论断已经过时。Norgen Biotek总RNA纯化试剂盒不仅避免Trizol带给实验者的健康损害,而且可以纯化出包括miroRNA在内的所有RNA。因此Norgen Biotek是一款真正意义上的绿色产品。
鉴于以上原因,Norgen被著名的microRNA表达谱分析公司Exiqon定为专有核酸提取供应商(请点击这里了解详情)。
更多关于Norgen 总RNA产品的特点介绍,请点击这里。
Norgen Biotek的RNA纯化产品包括:
RNA Kits |
产品 |
货号 |
规格 |
Total RNA Purification Kit | 17200 | 50 preps | |
Animal Tissue Total RNA Purification Kit | 25700 | 50 preps | |
Cytoplasmic & Nuclear RNA Purification Kit | 21000 | 50 preps | |
Soil DNA Isolation Kit | 26500 | 50 preps | |
Water RNA/DNA Purification Kit 0.22um | 26450 | 25 preps | |
Water RNA/DNA Purification Kit 0.45um | 26450 | 25 preps | |
Leukocyte RNA Purification Kit | 21200 | 50 preps | |
microRNA Purification Kit | 21300 | 25 preps | |
RNA/DNA/Protein Purification Kit | 23500 | 20 preps | |
RNA/Protein Purification Kit | 23000 | 20 preps | |
RNA CleanUp and Concentration Kit | 23600 | 50 preps | |
Urine Exfoliated Cell RNA Purification Kit | 22500 | 20 preps | |
Urine Bacteria RNA Purification Kit | 23400 | 20 preps | |
All-in-One Purification Kit | 24200 | 20 preps | |
FFPE RNA Purification Kit | 25300 | 50 preps | |
FFPE RNA/DNA Purification Kit | 25000 | 50 preps | |
Plant/Fungi RNA Purification Kit | 25800 | 50 preps | |
Plant RNA/DNA Purification Kit | 24400 | 50 preps | |
Total RNA Purification Kit (96-Well Format) | 24300 | 2 plates/192 preps | |
RBC Lysis Solution | 21201 | 90 mL |
产品说明书
Total RNA纯化试剂盒
Total RNA纯化试剂盒能够快速地从培养的细胞,组织样品,血液,细菌,酵母,真菌,以及植物中提取和纯化total RNA。
- 纯化多种RNA,包括大片段的mRNA和rRNA,以及microRNA和siRNA等小片段
- 操作快速简单,能够在20分钟内可以完成多达10个样品的分离和纯化,为你节省宝贵的实验时间
- 无需酚和氯仿的抽提过程,为你保持一个绿色健康的实验环境
- 可以从很少量(比如,一个细胞中)的样品中提取total RNA,是你再不用为少量的样品而发愁
- 所纯化的total RNA可以广泛的应用于下游操作中,例如,定量PCR(real-time PCR),northen-blotting,RNAase protection和引物延伸,以及涉及RNA的基因芯片研究
简单快速的操作流程,在很短的时间里提纯出Total RNA,为您节省了宝贵的时间和精力
Norgen的total RNA纯化试剂盒可以从E.Coli中提纯出高质量的RNA。和其他公司的类似产品相比,Norgen试剂盒可以把小片段的RNA成功的分离出来
(红色圆圈所包括的条带)。
高敏感性。从递减的 293细胞中分离出的total RNA,经过RT-PCR所产生的条带
Total RNA纯化试剂盒(96孔板):从96孔板中直接提取Total RNA
动物组织Total RNA纯化试剂盒:该产品能从所有动物组织中,包括富含纤维的肌肉和心脏中,提取总RNA。试剂盒里提供的Proteinase K可以有效地去除组织中的胶原,收缩蛋白,结缔组织。提纯出的RNA包括所有分子量大小的RNA,例如大mRNA,rRNA,以及microRNA,siRNA,并且不含有酚和氯仿等有害物质,可以直接用于下游实验,例如,Real time PCR,逆转录PCR,Northern blotting,RNAase protection,引物延伸和芯片检测。
microRNA纯化试剂盒: 能够从培养的细胞,小的组织样品,以及细菌中快速纯化小于200nt的全部小RNA分子,例如miRNA,siRNA,tRNA,和5S rRNA等。
- 操作快速简易。能够在30分钟内完成10个样品的提取和纯化
- 无需酚和氯仿的抽提过程,为你保持一个健康的实验环境
- 无大片段RNA和基因组DNA的污染• 所纯化的RNA可以广泛的应用于下游操作中,例如,Northern blotting,microarray analysis等。
简单快速的操作流程,使您在很短的时间里提纯出micro RNA
分类 | 货号 | 品名 | 规格 | 目录价 | 起订量 |
RNA Kits | 17200 | Total RNA Purification Kit | 50 preps | ¥2,940.00 | |
37500 | Total RNA Purification Kit | 100 preps | ¥5,523.00 | ||
24300 | Total RNA Purification Kit - 96 well format | 2 plates | ¥8,526.00 | ||
26800 | Total RNA Purification Maxi Kit - 8 preps | 8 preps | ¥1,764.00 | ||
48300 | Total RNA Purification Plus Kit (50) | 50 preps | ¥3,486.00 | ||
48400 | Total RNA Purification Plus Kit (100) | 100 preps | ¥6,132.00 | ||
25700 | Animal Tissue RNA Purification Kit | 50 preps | ¥4,042.60 | ||
35300 | Total RNA Purification Micro Kit - 50 preps | 50 preps | ¥3,465.00 | ||
36200 | Fatty Tissue RNA Purification Kit | 25 preps | ¥2,415.00 | ||
21000 | Cytoplasmic & Nuclear RNA Purification Kit | 50 preps | ¥3,675.00 | ||
37400 |
Cytoplasmic & Nuclear RNA Purification Kit | 100 preps | ¥6,993.00 | ||
21200 | Leukocyte RNA Purification Kit | 50 preps | ¥3,675.00 | ||
37800 | Leukocyte RNA Purification Kit - 96 Well | 2 plates | ¥9,187.60 | ||
23400 | Urine Bacteria RNA Purification Kit | 20 preps | ¥2,058.00 | ||
47200 | Urine Exosome RNA Purification Kit | 50 preps | ¥5,260.00 | ||
49200 | Plasma/Serum Exosome RNA Purification Kit | 50 preps | ¥5,260.00 | ||
22500 | Urine (exfoliated cell) RNA Purification Kit | 20 preps | ¥2,352.00 | ||
29000 | Urine microRNA Purification Kit | 25 preps | ¥2,940.00 | ||
21300 | microRNA Purification Kit | 25 preps | ¥2,415.00 | ||
24400 | Plant RNA/DNA Purification Kit | 50 preps | ¥2,940.00 | ||
25300 | FFPE RNA Purification Kit | 50 preps | ¥4,042.60 | ||
25400 | FFPE RNA Purification Kit (96-well) | 2 plates | ¥9,996.00 | ||
25000 | FFPE RNA/DNA Purification Kit | 50 preps | ¥3,675.00 | ||
23600 | RNA Cleanup and Concentration Kit | 50 preps | ¥2,940.00 | ||
43200 | RNA Cleanup and Concentration Kit | 100 preps | ¥5,523.00 | ||
25100 | RNA Cleanup and Concentration Kit 96 well | 2 plates | ¥8,526.00 | ||
21201 | RBC Lysis Solution | 90 mL | ¥735.00 | ||
21205 | RBC Lysis Solution | 500 mL | ¥3,675.00 | ||
21207 | RBC Lysis Solution | 1000 mL | ¥6,720.00 | ||
25800 | Plant/Fungi RNA Purification Kit | 50 preps | ¥3,675.00 | ||
31300 | Plant/Fungi RNA Purification Kit 96 preps | 96 preps | ¥7,056.00 | ||
31900 | Plant/Fungi RNA Purification Kit 96 well format | 2 plates | ¥9,261.00 | ||
42300 | Plant RNA Purification Kit - Maxi format | 8 preps | ¥2,719.60 | ||
24200 | All-in-One Purification Kit | 20 preps | ¥3,675.00 | ||
23500 | RNA/DNA/Protein Purification Kit - 20 Preps | 20 preps | ¥2,940.00 | ||
24000 | RNA/DNA/Protein Purification Kit - 50 Preps | 50 preps | ¥6,247.60 | ||
47800 | RNA/DNA/Protein Purification Plus Kit - 20 Preps | 20 preps | ¥3,206.00 | ||
47700 | RNA/DNA/Protein Purification Plus Kit - 50 Preps | 50 preps | ¥6,930.00 | ||
48600 | RNA/DNA Purification Kit - 20 preps | 20 preps | ¥2,716.00 | ||
48700 | RNA/DNA Purification Kit - 50 preps | 50 preps | ¥5,866.00 | ||
50300 | RNA/DNA Purification Micro Kit | 50 preps | ¥6,240.00 | ||
23000 | RNA/Protein Purification Kit - 20 preps | 20 preps | ¥2,415.00 | ||
24100 | RNA/Protein Purification Kit - 50 preps | 50 preps | ¥5,292.00 | ||
48100 | RNA/Protein Purification Plus Kit - 20 preps | 20 preps | ¥2,716.00 | ||
48200 | RNA/Protein Purification Plus Kit - 50 preps | 50 preps | ¥5,866.00 | ||
23800 | CleanAll Purification Kit | 20 preps | ¥2,940.00 | ||
26450 | Water RNA/DNA Purification Kit - 0.45 um | 25 preps | ¥5,544.00 | ||
26400 | Water RNA/DNA Purification Kit - 0.22 um | 25 preps | ¥5,817.00 | ||
29600 | Urine Total RNA Purification Maxi Kit - slurry format | variable | ¥5,817.00 | ||
32800 | Viroid RNA Purification Kit | 50 preps | ¥4,042.60 | ||
41900 | Viroid RNA Purification 96-Well Kit | 2 plates | ########## | ||
27700 | Soil RNA Purification Kit | 25 preps | ¥3,234.00 | ||
49400 | Stool RNA Purification Kit - 25 preps | 25 preps | ¥2,866.60 | ||
49500 | Stool RNA Purification Kit - 50 preps | 50 preps | ¥4,900.00 | ||
35800 | Yeast/Fungal RNA/DNA Purification Kit - 50 preps | 50 preps | ¥3,465.00 | ||
25710 | RNase-Free DNase I Kit | 50 reactions | ¥1,470.00 | ||
48900 | Blood RNA/DNA Preservation and Isolation Kit | 20 preps | ¥3,430.00 | ||
43400 | Preserved Blood RNA Purification Kit I (for use with TempusTM Blood RNA Tubes) | 50 preps | ¥5,250.00 | ||
43500 | Preserved Blood RNA Purification Kit II (for use with PaxgeneTM Blood RNA Tubes) | 50 preps | ¥5,250.00 | ||
Protein Kits | 10100 | ProteoSpin CBED Micro Kit | 25 preps | ¥2,940.00 | |
10400 | ProteoSpin CBED Micro Kit | 50 preps | ¥4,630.60 | ||
17000 | ProteoSpin CBED Maxi Kit | 4 preps | ¥1,617.00 | ||
10200 | ProteoSpin Detergent Clean-Up Micro Kit | 25 preps | ¥2,940.00 | ||
10500 | ProteoSpin Detergent Clean-Up Micro Kit | 50 preps | ¥4,630.60 | ||
17100 | ProteoSpin Detergent Clean-Up Maxi Kit | 4 preps | ¥1,617.00 | ||
10300 | ProteoSpin Inclusion Body Isolation Micro Kit | 20 preps | ¥3,255.00 | ||
10600 | ProteoSpin Inclusion Body Isolation Micro Kit | 50 preps | ¥5,218.60 | ||
17700 | ProteoSpin Inclusion Body Isolation Maxi Kit | 4 preps | ¥1,837.60 | ||
17300 | ProteoSpin Abundant Serum Protein Depletion Kit | 25 preps | ¥2,940.00 | ||
17400 | ProteoSpin Urine Protein Concentration Kit | 25 preps | ¥2,940.00 | ||
21600 | ProteoSpin Urine Protein Concentration MAXI Kit | 4 preps | ¥1,732.60 | ||
17500 | ProteoSpin On-Column Proteolytic Digestion Kit | 25 preps | ¥2,940.00 | ||
18700 | Inclusion Body Solubilization Reagent | 25 mL | ¥582.80 | ||
18701 | Inclusion Body Solubilization Reagent | 100 mL | ¥1,890.00 | ||
18800 | Cell Lysis Reagent | 100 mL | ¥1,023.80 | ||
18801 | Cell Lysis Reagent | 500 mL | ¥1,890.00 | ||
30300 | Detergent Free Total Protein Purification Kit | 25 preps | ¥2,940.00 | ||
22000 | ProteoSpin Total Protein Concentration Kit | 25 preps | ¥2,940.00 | ||
23300 | ProteoSpin Total Protein Detergent CleanUp Kit | 20 preps | ¥2,940.00 | ||
22800 | ProteoSpin Endotoxin Removal Micro Kit - For Proteins and Peptides | 20 preps | ¥2,667.00 | ||
22200 | ProteoSpin Endotoxin Removal Maxi Kit - For Proteins and Peptides | 4 preps | ¥1,638.00 | ||
43600 | ProteoSpin Endotoxin Removal Maxi Kit - For Proteins and Peptides | 24 preps | ¥9,072.00 | ||
DNA Kits | 13300 | Plasmid MiniPrep Kit | 50 preps | ¥1,102.60 | |
46500 | Plasmid MaxiPrep Kit (4 Preps) | 4 preps | ¥1,407.00 | ||
46600 | Plasmid MaxiPrep Kit (20 Preps) | 20 preps | ¥6,320.00 | ||
25900 | Plasmid MiniPrep Kit - 96 well x 1 | 1 plate | ¥3,241.40 | ||
43300 | Plasmid MiniPrep Kit - 96 well x 4 | 4 plates | ########## | ||
14400 | PCR Purification Kit | 50 preps | ¥1,176.00 | ||
45700 | PCR Purification Kit (250) | 250 preps | ¥5,586.00 | ||
24800 | PCR Purification Kit - 96 well format | 2 plates | ¥5,145.00 | ||
34400 | Sequencing Reaction Clean-Up Kit (96 well) | 2 plates | ¥5,145.00 | ||
34500 | Sequencing Reaction Clean-Up Kit | 50 preps | ¥2,058.00 | ||
13100 | DNA Gel Extraction Kit | 50 preps | ¥1,176.00 | ||
17900 | Bacterial Genomic DNA Isolation Kit | 50 preps | ¥1,470.00 | ||
18600 | Yeast Genomic DNA Isolation Kit | 50 preps | ¥1,470.00 | ||
26300 | Yeast Genomic DNA Isolation Kit - 96 Well Format | 2 plates | ¥7,276.60 | ||
27300 | Fungi/Yeast Genomic DNA Isolation Kit | 50 preps | ¥2,058.00 | ||
24700 | Genomic DNA Isolation Kit (50) | 50 preps | ¥1,470.00 | ||
17800 | Low Copy Plasmid MiniPrep Kit | 25 preps | ¥1,102.60 | ||
18050 | BAC DNA MiniPrep Kit | 50 preps (1.5 mL culture) | ¥1,680.00 | ||
18100 | Urine DNA Isolation Kit | 50 preps | ¥3,675.00 | ||
48800 | Urine DNA Isolation Kit (slurry format) | 50 preps | ¥6,300.00 | ||
50100 | Urine DNA Isolation Maxi Kit (slurry format) | 50 preps | ¥7,700.00 | ||
27100 | Urine DNA Isolation 96-Well Kit (Slurry Format) | 1 plate | ¥6,615.00 | ||
47050 | Urine DNA Isolation Kit for Exfoliated Cells or Bacteria | 50 preps | ¥2,060.00 | ||
22700 | Endotoxin Removal Kit (Mini) - For DNA | 20 preps | ¥2,667.00 | ||
21900 | Endotoxin Removal Kit (Maxi) - For DNA | 4 preps | ¥1,638.00 | ||
45400 | Saliva DNA Isolation Kit (50 Prep) | 50 preps | ¥1,750.00 | ||
35200 | Saliva DNA Isolation 96-Well Kit - 2 96-Well Plates, 192 Preps | 2 x 96 wells | ¥6,580.00 | ||
21500 | Milk Bacterial DNA Isolation Kit | 25 preps | ¥1,911.00 | ||
46700 | Phage DNA Isolation Kit | 25 preps | ¥2,415.00 | ||
46800 | Phage DNA Isolation Kit | 50 preps | ¥4,550.00 | ||
25600 | Direct Fungi DNA Isolation Kit | 25 preps | ¥2,058.00 | ||
47400 | FFPE DNA Isolation Kit | 50 preps | ¥2,600.00 | ||
26200 | Plant/Fungi DNA Isolation Kit | 50 preps | ¥2,352.00 | ||
26900 | Plant/Fungi DNA Isolation Kit (96-well) | 2 plates | ¥7,276.60 | ||
26500 | Soil DNA Isolation Kit | 50 preps | ¥2,940.00 | ||
49300 | Soil DNA Isolation Kit (High Humic Acid) | 50 preps | ¥3,080.00 | ||
43700 | Soil DNA Isolation 96-Well Kit (Slurry Format) | 1 x 96 wells | ¥9,660.00 | ||
27600 | Stool DNA Isolation Kit | 50 preps | ¥2,940.00 | ||
34100 | Oligo Clean-Up and Concentration Kit | 50 preps | ¥2,940.00 | ||
36000 | Dried Blood Spot (DBS) Genomic DNA Isolation Kit | 50 preps | ¥2,583.00 | ||
35900 | Dried Blood Spot (DBS) Genomic DNA Isolation Kit (For Resource Limited Settings) | 50 preps | ¥2,940.00 | ||
44800 | Milk DNA Preservation and Isolation Kit | 25 preps | ¥2,940.00 | ||
DNA Ladders | 11200 | Mini Sizer 50bp DNA Ladder | 100 loads | ¥735.00 | |
13525 | 50 bp DNA Ladder | 100 loads | ¥735.00 | ||
11300 | PCR Ranger 100bp DNA Ladder | 100 loads | ¥735.00 | ||
11400 | PCR Sizer 100bp DNA Ladder | 100 loads | ¥735.00 | ||
12800 | FastRunner DNA Ladder | 100 loads | ¥735.00 | ||
11500 | LowRanger 100bp DNA Ladder | 100 loads | ¥735.00 | ||
11600 | Clone Sizer 100bp DNA Ladder | 100 loads | ¥735.00 | ||
11700 | MidRanger 1kb DNA Ladder | 100 loads | ¥735.00 | ||
11800 | FullRanger 100bp DNA Ladder | 100 loads | ¥735.00 | ||
11900 | HighRanger 1kb DNA Ladder | 100 loads | ¥735.00 | ||
12000 | HighRanger Plus 100bp DNA Ladder | 100 loads | ¥735.00 | ||
12100 | UltraRanger 1kb DNA Ladder | 100 loads | ¥735.00 | ||
11215 | Mini Sizer 50bp DNA Ladder | 5 x 100 loads | ¥3,360.00 | 10 | |
13527 | 50 bp DNA Ladder | 5 x 100 loads | ¥3,360.00 | 10 | |
11315 | PCR Ranger 100bp DNA Ladder | 5 x 100 loads | ¥3,360.00 | 10 | |
11415 | PCR Sizer 100bp DNA Ladder | 5 x 100 loads | ¥3,360.00 | 10 | |
12815 | FastRunner DNA Ladder | 5 x 100 loads | ¥3,360.00 | 10 | |
11515 | LowRanger 100bp DNA Ladder | 5 x 100 loads | ¥3,360.00 | 10 | |
11615 | Clone Sizer 100bp DNA Ladder | 5 x 100 loads | ¥3,360.00 | 10 | |
11715 | MidRanger 1kb DNA Ladder | 5 x 100 loads | ¥3,360.00 | 10 | |
11815 | FullRanger 100bp DNA Ladder | 5 x 100 loads | ¥3,360.00 | 10 | |
11915 | HighRanger 1kb DNA Ladder | 5 x 100 loads | ¥3,360.00 | 10 | |
12015 | HighRanger Plus 100bp DNA Ladder | 5 x 100 loads | ¥3,360.00 | 10 | |
12115 | UltraRanger 1kb DNA Ladder | 5 x 100 loads | ¥3,360.00 | 10 | |
RNA Ladders | 15002 | 100b RNA Ladder | 50 loads | ¥1,134.00 | |
15003 | 1kb RNA Ladder | 50 loads | ¥1,134.00 | ||
15006 |
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蚂蚁淘 2019-04-25
全部回答 (共 2 条)
Norgen Biotek 商品列表
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