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蚂蚁淘在线 / 品牌中心 / Norgen Biotek / Norgen Biotek /Blood DNA Isolation Mini Kit (Cat. 46300, 46380)/50Preps/46300
商品描述
Blood DNA Isolation Mini Kit
Norgen’s Blood DNA Isolation Mini Kit is designed for the rapid preparation of DNA from up to 200 µL of whole blood using a rapid spin column protocol. DNA can be isolated from the blood of various species, including humans. Preparation time for a single sample is less than 30 minutes and each kit contains sufficient materials for 50 preparations. The kit isolates genomic DNA, mitochondrial DNA, viral DNA and bacterial DNA from blood samples. The purified DNA is of excellent quality and yield and completely compatible with downstream applications including PCR, qPCR, Sequencing, Genotyping, Virus Detection, Southern Blot analysis.
Figure 1.High Yields of DNA Isolated from 20 µL to 200 µL of Whole Blood. DNAwas isolated from 20, 50, 100 and 200 µLofwhole blood using Norgen's Blood DNAIsolation Mini Kit and a leading competitor's kit. Following isolation, 15 µLfrom each 200 µL elution was loaded on 1% TAE agarose gel. Norgen's Blood DNA Isolation Mini Kitdemonstrated a better DNA yield than the leading competitor's kit. Lane M: Norgens UltraRanger 1kb DNA Ladder.]">
Figure 1.High Yields of DNA
Figure 2.Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction. DNAwas isolated from 20, 50, 100 and 200µLof whole human blood using Norgen's Blood DNAIsolation Mini Kit (Blue) and a leading competitor's kit (Red).Nine µL of the DNA from each 200 µL elution was used in a real-time PCR reaction (total reaction volume of 20 µL) with GAPDH TaqMan probe and primers. The real-time PCR was successful in amplifying the GAPDH gene, indicating that the DNA is of a high quality and can be used in sensitive downstream applications. Furthermore, Norgen-isolated DNA was amplified with a lower Ct value, indicating the higher yield and purity of DNAisolated using Norgen's kit. The black line is a no-template control.]">
Figure 2.Amplification of DNA by Real-Time PCR
Figure 3. Purified DNA Can be Amplified in a Real-time PCR (TaqMan) Reaction.DNAwas isolated from 20, 50, 100 and 200µLof whole human blood using Norgen's Blood DNAIsolation Mini Kit (Blue) and a leading competitor's kit (Red).Nine µL of the DNA from each 200 µL of elution was used in a real-time PCR reaction (total reaction volume of 20 µL) with GAPDH TaqMan probe and primers. The real-time PCR was successful in amplifying the GAPDH gene, with a linear decrease in Ct value with the increase in blood input volume, indicating that the DNA is of a high quality and can be used in sensitive downstream applications. Furthermore, Norgen-isolated DNA was amplified with a lower Ct value from all DNA isolated from the different blood input volumes, indicating the higher yield and purity of DNAisolated using Norgen's kit.]">
Figure 3. Amplification of DNA by Real-Time PCR
