Cyanine3.5 NHS ester (an analog of Cy3.5® NHS ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides.
Cyanine3.5 NHS ester can replace NHS esters of Cy3.5®, Alexa Fluor 594, DyLight 594.
![](\"https://www.ebiomall.com/images/201709/cy35-nhs-ester.png\")
Download in ChemDraw formatGeneral properties
| Appearance: | dark purple powder |
| Molecular weight: | 690.27 |
| Molecular formula: | C42H44ClN3O4 |
| Solubility: | soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water |
| Quality control: | NMR 1H and HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Spectral properties
| Excitation maximum, nm: | 591 |
| ε, L⋅mol−1⋅cm−1: | 116000 |
| Emission maximum, nm: | 604 |
| Fluorescence quantum yield: | 0.35 |
| CF260: | 0.29 |
| CF280: | 0.22 |
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