Can be oxidized by Renilla luciferases (Rluc) but not Gaussia luciferase. It is used for real-time detection of calcium ions, gene reporter assays and a favored substrate for BRET. This derivate has an emission maximum around 400 nm and therefore minimal interference with the emission of the GFP acceptor.
| Compound ID: | Coelenterazine 400a, 1-bisdeoxycoelenterazine, di-dehydrocoelenterazine, DeepBlueCTM |
| CAS# | 70217-82-2 |
| IUPAC Name: | 2,8-DIBENZYL-6-PHENYL-IMIDAZO[1,2A]PYRAZIN-3-(7H)- 1; BISDEOXYCOELENTERAZINE |
| Molecular Formula: | C26H21N3O |
| MW: | 391.5 |
| Structure: |
How to Use Coelenterazine 400a
Certificate of Analysis HPLC MSDS NMR
The luciferin is shipped as filtered, lyophilized, batch controlled substratein 500 µg aliquots (1 mg = 2x 500 µg) and in 5 mgs and 10mgs(2 x5mgs) aliquots.
The advantages of lyophilized luciferins are:
- pre-aliquoted in small amounts, fresh substrate for every set of experiments
- long shelf-life (packed under Argon)
- faster to dissolve due to its fine crystal structure
- consistent quality between aliquotes
Shipping: via FedEx or UPS at RT, overnight or 2nd delivery. Store at -80°C. Protect from light.
>>> 更多资讯详情请访问蚂蚁淘商城
