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商品描述
HighpuritydyedandcrosslinkedGalactazymetabletsforthemeasurementofenzymeactivity,forresearch,biochemicalenzymeassaysandinvitrodiagnosticanalysis.
Fortheassayofendo-1,4-β-D-galactanase.ContainingAZCL-Galactan(potato).
Theβ-1,4‐endogalactanaseAgenefromAspergillusnigerisspecificallyinducedonarABInoseandgalacturonicacidandplaysanimportantroleinthedegradationofpectichairyregions.
deVries,R.P.,Pařenicová,L.,Hinz,S.W.A.,Kester,H.C.M.,Beldman,G.,Benen,J.A.E.&Visser,J.(2002).EuropeanJournalofBiochemistry,269(20),4985-4993.
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TheAspergillusnigerβ-1,4-endogalactanaseencodinggene(galA)wasclonedandcharacterized.TheexpressionofgalAinA.nigerwasonlydetectedinthepresenceofsugarbeetpectin,D-galacturonicacidandL-arabinose,suggestingthatgalAiscoregulatedwithboththepectinolyticgenesaswellasthearabinanolyticgenes.Thecorrespondingenzyme,endogalactanaseA(GALA),containsbothactivesiteresiduesidentifiedpreviouslyforthePseudomonasfluorescensβ-1,4-endogalactanase.ThegalAgenewasoverexpressedtofacilitatepurificationofGALA.Theenzymehasamolecularmassof48.5kDaandapHoptimumbetween4and4.5.Incubationsofarabinogalactansofpotato,onionandsoywithGALAresultedinitiallyinthereleaseofD-galactotrioseandD-galactotetraose,whereasprolongedincubationresultedinD-galactoseandD-galactobiose,predominantly.MALDI-TOFanalysisrevealedthereleaseofL-arabinosesubstitutedD-galactooligosaccharidesfromsoyarabinogalactan.Thisisthefirstreportoftheabilityofaβ-1,4-endogalactanasetoreleasesubstitutedD-galacto-oligosaccharides.GALAwasnotactivetowardsD-galacto-oligosaccharidesthatweresubstitutedwithD-glucoseatthereducingend.