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商品描述
Highpuritydyedandcrosslinked1,3-β-Glucazymetabletsforthemeasurementofenzymeactivity,forresearch,biochemicalenzymeassaysandinvitrodiagnosticanalysis.
Fortheassayofendo-1,3-β-D-glucanase(laminarinase).ContainingAZCL-Pachyman;a1,3-β-D-glucan.
Lentinulaedodestlg1encodesathaumatin-likeproteinthatisinvolvedinlentinandegradationandfruitingbodysenescence.
Sakamoto,Y.,Watanabe,H.,Nagai,M.,Nakade,K.,Takahashi,M.&Sato,T.(2006).PlantPhysiology,141(2),793-801.
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LentinanisanantitumorproductthatispurifiedfromfreshLentinulaedodesfruitingbodies.Itisacellwallcomponent,comprisingβ-1,3-glucanwithβ-1,6-linkedbranches,whichbecomesdegradedduringpostharvestpreservationasaresultofincreasedglucanaseactivity.Inthisstudy,weusedN-terminalaminoacidsequencetoisolatetlg1,ageneencodingathaumatin-like(TL)proteininL.edodes.TheCDNAclonewasapproximately1.0kbwhereasthegenomicsequencewas2.1kb,andcomparisonofthetwoindicatedthattlg1contains12introns.Thetlg1geneproduct(TLG1)waspredictedtocomprise240aminoacids,withamolecularmassof25kDandisoelectricpointvalueof3.5.Theputativeaminoacidsequenceexhibitsapproximately40%identitywithplantTLproteins,andafungalgenomedatabasesearchrevealedthattheseTLproteinsareconservedinmanyfungiincludingthebasidiomycotaandascomycota.Transcriptionoftlg1wasnotdetectedinvegetativemyceliumoryoungandfreshmushrooms.However,transcriptionincreasedfollowingharvest.Western-blotanalysisdemonstratedariseinTLG1levelsfollowingharvestandsporediffusion.TLG1expressedinEscherichiacoliandAspergillusoryzaeexhibitedβ-1,3-glucanaseactivityand,whenpurifiedfromtheL.edodesfruitingbody,demonstratedlentinandegrADIngactivity.Thus,wesuggestthatTLG1isinvolvedinlentinanandcellwalldegradationduringsenescencefollowingharvestandsporediffusion.


