品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
CAS:1398-61-4
MolecularFormula:(C8H13NO5)n
Highpurityborohydridereducedcolloidalchitinforuseinresearch,biochemicalenzymeassaysandinvitro diagnosticanalysis.
Thissubstrateisrecommendedfortheassayofendo-chitinaseusingreducingsugarmethods.
Synonyms:beta-(1,4)-2-Acetamido-2-deoxy-D-glucose;Poly-(1,4)-β-N-acetyl-D-glucosamine.
StABIlity:>10yearsatroomtemperature.
EquivalenttoSigmaproductChitinfromshrimp(#C9752).
AchitindeacetylaseofPodosporaanserinahastwofunctionalchitinbindingdomainsandauniquemodeofaction.
Hoßbach,J.,Bußwinkel,F.,Kranz,A.,Wattjes,J.,Cord-Landwehr,S.&Moerschbacher,B.M.(2017).CarbohydratePolymers,InPress.
LinktoArticle
ReadAbstract
Chitosanisastructurallydiversebiopolymerthatiscommerciallyderivedfromchitinbychemicalprocessing,butchitindeacetylases(CDAs)potentiallyofferasustainableandmorecontrollableapproachallowingtheproductionofchitosanswithtailoredstructuresandBIOLOGicalactivities.WeinvestigatedtheCDAfromPodosporaanserina(PaCDA)whichiscloselyrelatedtoColletotrichumlindemuthianumCDAinthecatalyticdomain,butuniqueinhavingtwochitin-bindingdomains.WeproducedrecombinantPaCDAinHansenulapolymorphaforbiochemicalcharacterizationandfoundthatthecatalyticdomainofPaCDAisalsofunctionallysimilartoC.lindemuthianumCDA,thoughdifferingindetail.Whenstudyingtheenzyme’smodeofactiononchitinoligomersbyquantitativemass-spectrometricsequencing,wefoundalmostallpossIBLesequencesuptofulldeacetylationbutwithaclearpreferenceforspecificproducts.DeletionmuteinslackingoneorbothCBDsconfirmedtheirproposedfunctioninsupportingtheenzymaticconversionoftheinsolublesubstratecolloidalchitin.


