I-5 Hi-Fi DNA HotStart Polymerase is a high-fidelity and fast DNA polymerase.
General DescriptionI-5 Hi-Fi DNA HotStart Polymerase is an ultra-fast and high-fidelity DNA polymerase. It provides robust amplification of difficult templates including plasmids, BACS, genomic DNA, and lambda DNA. It allows the amplification of up to 8kb human genomic DNA and up to 21kb lambda DNA. It has an extension speed of 1 kb / 10-15 seconds depending on template type. This allows users to save time by speeding up PCR reactions and provides higher fidelity than Taq or Pfu. The enzyme contains a HotStart mechanism that inactivates the enzyme until it is heated. This allows users to setup PCR reactions at room temperature without worrying about primer dimers or non-specific preamplification.Feature• Fast – 4X faster than hot start Taq• Robust – high inhibitor tolerance• High yields – high efficiency• Long PCR products Source E. coli Applications Hot Start PCRRoutine PCRFast PCRHigh throughput PCRGenotyping Supplied with 5X Reaction Buffer (1ml, 5X MgCl2 included ) 50mM MgCl2 (1ml) (for optimization) Supplied in (buffer description) 20mM Tris-HCl, 100mM KCl, 1mM DTT, 0.1mM EDTA, 200ug/ml BSA, 50% glycerol, 1X stabilizer, pH 8.0 @ 25 C Storage Condition -20ºC Unit Definition One unit is the amount of enzyme that incorporates 5 nmol of dNTPs into acid insoluble material in 15 minutes at 72°C. Reference Frey, B. and Suppman, B. (1995). BioChemica. 2, 34-35.Chester, N. and Marshak, D.R. (1993). Analytical Biochemistry. 209, 284-290. Shipping Conditiondry ice User protocol
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