Description:Exonuclease III (E. coli) is a 3\' -> 5\' exonuclease which acts by digesting one strand of a dsDNA duplex at a time or digesting the RNA strand of an RNA-DNA heteroduplex^(1,2). Exonuclease III (E. coli) breaks phosphodiester bonds on the 5\'-side of AP sites in both dsDNA and ssDNA⁽³⁾. It removes 3\'-terminal groups on dsDNA⁽³⁾, increases MutY turnover⁽⁴⁾, and efficiently degrades 3\'-recessed but not 3\' protruding DNA ends (creating 5\'-overhangs)⁽⁵⁾. Exonuclease III (E. coli) removes a limited number of nucleotides per binding event, resulting in coordinated progressive deletions within the population of DNA molecules⁽¹⁾.Specific Activity: 150,000 U/mgApplication:Degrades excess single-stranded primer oligonucleotides from a reaction mixture containing double-stranded extension products.Source:Purified from a strain of E. coli that expresses the recombinant Exonuclease III gene.Supplied in:25 mM Tris-HCl50 mM KCl1.0 mM DTT0.1% mM EDTA50% GlycerolpH 8.0 @ 25°CSupplied with: 10x Yellow Buffer10x Yellow Buffer:100 mM Bis-Tris-Propane100 mM MgCl₂10 mM DTTpH 7.0 @ 25°CUnit Definition:One unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble total nucleotide in 30 minutes at 37°C.Recommended Storage Condition: -20°CReferences:1. Linn, S. M. (1982) Nucleases, pp. 291-309, Cold Spring Harbor Laboratory Press.2. Shida, T., et al. (1996) Nucl. Acids Res. 24 (22), 4572-4576.3. Doetsch, P. W. (1990) Mutat. Res. 236 (2-3), 173-201.4. Pope, M. A., et al. (2002) J. Biol. Chem. 277 (25), 22605-22615.5. Henikoff, S. (1984) Gene 28, 351-359.