Description:Inorganic pyrophosphatase (PPase) is ubiquitous in nature and plays an important role in energy metabolism, providing a thermodynamic pull for biosynthetic reactions such as protein, RNA, and DNA synthesis. Escherichia coli K-12 gene ppa encoding inorganic pyrophosphatase (PPase) was cloned and sequenced. The 5\'-end of the ppa mRNA was identified by primer extension mapping.Source:E. coli strain carrying a plasmid encoding pyrophosphatase from E. coli K-12.Application:- Role in protein, RNA, and DNA synthesis- Catalyzing the reaction PPi + H₂O —> 2P_iUnit Definition:One unit will release 1.0 µmole of inorganic orthophosphate per minute at pH 8.5 at 37°C.

Specific Activity:  3,500 U/mgRecommended Storage Condition: -20°CReference:Kornberg, A. 1962. On the metabolic significance of phosphorolytic and pyrophosphorolytic reactions, p. 251-264. In H. Kasha and P. Pullman (ed.), Horizons in biochemistry. Academic Press, New York.