DescriptionHigh yields, high efficiency, and no heat shock necessary.

Application

• Ideal for expressing proteins in E. coli.
• BL21(DE3) cells carry the lambda DE3 lysogen.

Recommended torage conditionThis product should be stored at -80°C. Thaw on ice only before use. Do not re-freeze.Recommended reaction conditions

• Mix DNA with competent cells
• Let stand on bench for 5 minutes
• Direct plate

GenotypeF– ompT gal dcm lon hsdSB(rB- mB-) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5])ReferenceMiroux B, Walker JE. J. Mol. Biol. (1996) 260, 289-298.

Competent Cell Type:Chemically

Transformation Efficiency (cfu/μg):>1x10⁸

Blue-Whiet Screening:No

Strain:B

Reduces Recombination:No

Cloning Methylated DNA:No

Improves Plasmid Quality:No

Preparing Unmethylated DNA:Not suitable

T1 Phage Resistant:No

RecA Deficient:No