Description: T4 RNA Ligase 2, also known as T4 Rnl2 (gp24.1), catalyzes both inter- and intramolecular formation of phosphodiester bonds between 5\'-phosphate and 3\'-hydroxyl termini of oligonucleotides, single-stranded RNA and DNA. Unlike T4 RNA Ligase 1, T4 RNA Ligase 2 is much more active on joining nicks on double stranded RNA than on joining the ends of single-stranded RNA. The enzyme requires an adjacent 5\'-phosphate and 3\'-OH for ligation. The enzyme can also ligate the 3\'-OH of RNA to the 5\'-phosphate of DNA in a double stranded structure.

Source:An E. coli strain that carries the T4 RNA Ligase 2 gene (I. Schildkraut).Application:- Ligates a nick in dsRNA- Ligates the 3\'-OH of RNA to the 5\'-phosphate of DNA in a double stranded structureReagents Supplied: T4 Rnl2 Reaction BufferReaction Conditions: 1x T4 Rnl2 Reaction BufferIncubate at 37°C10x T4 RNA Ligase 2 Reaction Buffer:500 mM Tris-HCl20 mM MgCl₂10 mM DTT4 mM ATPpH 7.5 @ 25°CUnit Definition:One unit is defined as the amount of enzyme required to ligate 0.4 µg of an equimolar mix of a 23-mer and 17-mer RNAs in a total reaction volume of 20 µl in 30 minutes at 37°C.Unit Assay Conditions:1x T4 Rnl2 Reaction Buffer and 0.4 µg of an equimolar mix of the 23-mer and 17-mer RNAs. After incubation at 37°C for 30 minutes, the ligated product is detected on a 15% polyacrylamide gel.

Specific Activity: >40,000U/mg 

Storage Conditions:10 mM Tris-HCl50 mM KCl35 mM (NH₄)₂SO₄0.1 mM DTT0.1 mM EDTA50% GlycerolpH 7.5 @ 25°CRecommended Storage Condition: -20°C