Description:Ribonuclease III (Rnase III) is an endoribunuclease that cleaves double-stranded RNA, resulting in 12-15 bp dsRNA fragments with 2-base, 3’-overhangs, and 5\' phosphate and 3\' hydroxyl termini.  

Source:Ribonuclease III (RNase III) from E. coli is an endoribonuclease that specifically digests dsRNA to dsRNA fragments that have two-base, 3\' overhangs.Application:- Digestion of dsRNA to short fragments- RNA structure, processing, and maturation studiesUnit Definition:1 unit of the Rnase III digests 1 µg of dsRNA in 20 minutes at 37°CSupplied With:10X RNase III Reaction Buffer10X RNase III Reaction Buffer:500 mM NaCl100 mM Tris pH 7.9100 mM MgCl₂10 mM DTTStorage Buffer:Supplied in 50% Glycerol, 50 mM Tris-HCl (pH 7.5),100 mM NaCl, 0.1 mM EDTA.Recommended Storage Condition: -20°C