WesternBlottingHRPSubstrate
lumigenECLPlus(PS-3)isanacridan-basedtechnologychemiluminescentandchemifluorescentsubstrateforsensitivedetectionofWesternblottedproteinsthatareboundtohorserADIshperoxidase(HRP)labeledantibodies.
- Highlysensitive - approximately5pgperbandoftargetproteinonWesternblot
- Sustainedluminescence-signalissustainedforoverseveralhours
- Dualsignalfunctionality-chemiluminescentandchemifluorescentsignalsaregeneratedsimultaneouslyallowingdetectiononx-rayfilmorwithadigitalimager
LumigenECLPlusTechnology
LumigenECLPlusutilizesauniqueacridanbasedtechnologyforthechemiluminescentandfluorescentdetectionofhorseradishperoxidase(HRP)conjugates.ThereactioninvolvestheenzymaticgenerationofthousandsofacridiniumesterintermediatesperminutewhichreactwithperoxideatalkalinepHtoproducesustainedhigh-intensitychemiluminescence.Additionally,thereactionend-productexhibitsalong-lastingfluorescentsignalwhichcanbedetectedonaCCDimagingdevice.