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蚂蚁淘在线 / 品牌中心 / Kapa biosystems / Kapa biosystems/KAPA Stranded RNA-Seq with RiboErase/KK8710/24 adapters x 40 µl each

Kapa biosystems/KAPA Stranded RNA-Seq with RiboErase/KK8710/24 adapters x 40 µl each

价格
￥17280.00
货号：KK8710
浏览量：127
品牌：Kapa biosystems
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商品描述

Description:

KAPASingle-IndexedAdapterKit,SetA+B(1.5µM)

KAPAStrandedRNA-SeqwithRiboErase

Evolvedtofocus.

TheKAPAStrandedRNA-SeqKitwithRiboEraseoffersahigh-quality,comprehensivesolutionfortranscriptomesequencing.Byutilizingatargetedenzymaticmethodfordepletion,ourworkflowenablessuperiorreductionofribosomalRNA(rRNA)andamorecompleterepresentationofthetranscriptome,includingprecursormRNAsandnon-codingRNA(ncRNA).KitsalsocontainKAPAHiFiforhigh-efficiencyandlow-biaslibraryamplification,andincludeastreamlined,“with-bead”protocol.

  • Upto99.98%rRNAdepletion*
  • FlexIBLeinputof100ng–1μgtotalRNAfromhuman,mouse,orratspecies*
  • Robustandreproducibleacrossvariousinputamounts
  • Automation-friendlyworkflow

NEW!KAPADual-IndexedAdapterKitsarenowavailable.FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapterandBeadCalculator.

DownloadourAdapterandBeadCalculator

 

*Dataonfile.
ForResearchUseOnly.Notforuseindiagnosticprocedures.

ProductHighlights

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IndustryleADIngrRNAdepletion

  • SuperiorrRNAdepletionfromhigh-qualityandFFPEsamples*
  • MoreeconomicalNGSsequencingduetodecreasedrRNAreads,providingdeepersequencingoftranscriptsofinterest
High-quality sequencing data obtained from KAPA Stranded RNA-Seq Kit with RiboErase when compared to the equivalent Illumina or NEB kits. With equivalent mapping rates, KAPA Stranded RNA-Seq Kit with RiboErase provides lower duplication rates, better coverage uniformity and consistently detects more unique transcripts and genes. This enables a more sensitive detection of the expressed genes within the sample. Data on file.
Improved coverage of GC-rich transcripts. IGV image showing coverage and splice junction tracks of the haemoglobin alpha transcript (HBA1) which has a GC content of 62.8%. The GC content track shows GC-rich regions in red, and AT-rich regions in blue. More even coverage, improved splice-site recognition, and more representative plot compared to the undepleted sample (red) is seen when libraries are prepared with the KAPA Stranded RNA-Seq Kit with RiboErase (green) compared to the equivalent Illumina (orange) or NEB (blue) kits. Data on file.
Better detection of low-abundance transcripts. IGV image showing coverage and splice junction tracks of MALAT1, a low abundance long non-coding RNA transcript, which is more comprehensively covered with use of KAPA Stranded RNA-Seq Kit with RiboErase (green) for library preparation. In comparison, Illumina Ribo-Zero Gold (orange) and NEBNext (blue) show less even coverage of this transcript. Data on file.
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Unsurpassedsequencingdataquality

  • Detectionofmoregenesanduniquetranscripts
  • Accurateandclearidentificationofsplicesitesandalternativegenesplicing
  • ImprovedcoverageenablingbetterdetectionofdifficultandGC-richtranscripts
Hierarchically clustered heatmap showing the correlations of gene expression levels between different laboratories and RNA input amounts. All correlations were greater than 0.93, with technical replicates produced in the same lab exceeding 0.98. Generally, all libraries from a single lab, including those produced from different input amounts, clustered together. The data indicates that the KAPA Stranded RNA-Seq Kit with RiboErase delivers robust, reproducible, and unbiased gene expression data from a wide range of input amounts. Data on file.
0.973)whicharebetterthanlibrariespreparedfromtheequivalentkitsfromIlluminaorNEB.Dataonfile.">Correlation of gene expression levels between replicate samples. Libraries prepared using 100 ng of UHR Total RNA with the KAPA Stranded RNA-Seq Kit with RiboErase show a very high correlation (R2>0.973)whicharebetterthanlibrariespreparedfromtheequivalentkitsfromIlluminaorNEB.Dataonfile.
0.973)whicharebetterthanlibrariespreparedfromtheequivalentkitsfromIlluminaorNEB.Dataonfile.">Correlation of gene expression levels between replicate samples. Libraries prepared using 100 ng of UHR Total RNA with the KAPA Stranded RNA-Seq Kit with RiboErase show a very high correlation (R2>0.973)whicharebetterthanlibrariespreparedfromtheequivalentkitsfromIlluminaorNEB.Dataonfile.
0.973)whicharebetterthanlibrariespreparedfromtheequivalentkitsfromIlluminaorNEB.Dataonfile.">Correlation of gene expression levels between replicate samples. Libraries prepared using 100 ng of UHR Total RNA with the KAPA Stranded RNA-Seq Kit with RiboErase show a very high correlation (R2>0.973)whicharebetterthanlibrariespreparedfromtheequivalentkitsfromIlluminaorNEB.Dataonfile.
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Highlyreproduciblesequencingresults

  • Highcorrelationevenbetweendifferenttestingconditions
  • Lowsample-to-samplevariationformorereliableresults
Improved coverage of the whole transcript. The housekeeping gene Beta-Actin has improved coverage at the 3’ and 5’ regions using the KAPA Stranded RNA-Seq Kit with RiboErase (green) when compared to the equivalent Illumina Ribo-Zero Gold (orange) and NEBNext (blue) kits. Data on file.
Minimal positional bias. With 100 ng of high-quality RNA input, 3
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Improvedcoverageuniformity

  • Uniformdistributionofreadsovereachtranscript
  • Minimal5’–3’biasacrosstranscripts


*Dataonfile.

RelatedProducts

Areyousequencinglow-input,FFPEorhighqualityDNA? RNA? CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:

KAPA Stranded mRNA-Seq Kits

KAPAStrandedmRNA-SeqKits

KAPA RNA HyperPrep Kits with RiboErase

KAPARNAHyperPrepKitswithRiboErase

KAPA mRNA HyperPrep Kits

KAPAmRNAHyperPrepKits

Library Amplification

KAPALibraryAmplificationKits

Library Preparation

KAPAHyperPrepKits

Library Quantification

KAPALibraryQuantificationKits

Applications:

Applications
  • WholeTranscriptome
  • GeneExpression
  • TranscriptDiscoveryandAnnotation
  • TotalRNA-Seq

KitSpecificationsandContents/Storage:

KitSpecificationsandContents/Storage

Kitscanbestoredforupto10monthsat-20ºC.

KitscontainKAPAHybridizationBuffer,KAPAHybridizationOligos(Human/Mouse/Rat),KAPADepletionBuffer,KAPARNaseH,KAPADNaseBuffer,KAPADnase,KAPAFragment,PrimeandEluteBuffer(2X),KAPA1stStrandSynthesisBuffer,KAPAScript,KAPA2ndStrandMarkingBuffer,KAPA2ndStrandSynthesisEnzymeMix,KAPAA-TailingBuffer(10X),KAPAA-TailingEnzyme,KAPALigationBuffer(5X),KAPADNALigase,KAPAPEG/NaClSPRISolution,KAPALibraryAmplificationPrimerMix(10X)andKAPAHiFiHotStartReadyMix(2X).

Components

RIBO-Component Chart

Specifications

Spec
Description
CompatiblePlatform
IlluminaHiSeq®,NextSeq®andMiSeq®
LibraryType
RNA
StartingMaterial
High-qualityTotalRNA
InputAmount
100ng-1µg
Kapa biosystems表观遗传分析基于阵列的方法和基于下一代测序（NGS）的方法都用于研究表观遗传修饰。有三种基于NGS的常见方法可用于表观遗传分析：甲基序列，ChIP序列和ATAC序列。Methyl-seq   用单核苷酸分辨率研究基因组的甲基化状态。该方法采用亚硫酸氢盐处理，可将胞嘧啶残基转化为尿嘧啶，而甲基化残基则保持不变。已经开发了几种甲基序列策略，包括全基因组亚硫酸氢盐测序（WGBS）和简化表示的亚硫酸氢盐测序（RRBS），这丰富了CpG岛。ChIP-seq将染色质免疫沉淀（ChIP）与NGS结合使用，以鉴定整个基因组中与DNA相关的蛋白质的结合位点，通常用于绘制组蛋白修饰和转录因子。该方法依靠靶向抗体的选择来富集与特定蛋白质结合的目标DNA片段。ATAC-seq是用于转座酶可及的染色质测序的一种测定方法，可确定染色质可及性的区域并绘制DNA结合蛋白的图谱，以鉴定活性启动子，增强子和其他顺式调控元件。该方法通过生成具有50,000个细胞的测序文库，已经改变了基因调控的分析方法。由于表观遗传学分析通常涉及超低输入DNA，因此从有限的材料构建高质量文库至关重要。罗氏测序解决方案提供了许多用于表观遗传工作流程的目标富集，文库制备和优化文库质量的解决方案。SeqCap®Epi靶标富集试剂盒可通过单碱基分辨率富集用于DNA甲基化评估的靶标。的  KAPA HyperPrep套件  理想地适合于这两个芯片起和甲基SEQ应用，因为它使接头连接的文库和扩增低偏压的更高的产率。这意味着更高的文库多样性，更低的重复率和更统一的覆盖率，尤其是对于低输入样本而言。对于甲基序列研究，  KAPA HiFi Uracil + 由于对尿嘧啶残基具有耐受性，HotStart DNA聚合酶对于亚硫酸氢盐转化的文库的扩增至关重要。KAPA HiFi HotStart ReadyMix可用于扩增ATAC-seq和ChIP-seq库，从而改善序列覆盖范围并减少偏差。