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蚂蚁淘在线 / 品牌中心 / Kapa biosystems / Kapa biosystems/Library Preparation for Illumina/KK8001/30 mL

Kapa biosystems/Library Preparation for Illumina/KK8001/30 mL

价格
￥9000.00
货号：KK8001
浏览量：127
品牌：Kapa biosystems
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商品描述

Description:

KAPAPureBeads(30mL)

LibraryPreparationforIllumina

It’scomplex,butwehaveitcovered.

KAPADNALibraryPreparationKitsforIllumina®sequencingplatformscontainevolvedandoptimallyformulatedenzymesthatenablethehighestoverallcoveragefromtheleastamountoftotalsequencing.

KitsareoptimizedtoachievesignificantlyhigherlibraryyieldsandcontainKAPAHiFiforhigh-fidelity,high-efficiencyandlow-biaslibraryamplification.Thisensuresthehighestlibraryandsequencedataquality,particularlyfromlow-inputanddifficultsamplessuchasFFPEandChIPDNA.

NEW!KAPADual-IndexedAdapterKitsarenowavailable.FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapterandBeadCalculator.

DownloadourKAPA AdapterandBeadCalculator

 

ForResearchUseOnly.Notforuseindiagnosticprocedures.

ProductHighlights

Key sequencing metrics (library diversity, left and duplication rates, right) for libraries prepared for exome capture (SureSelect All Human Exome V5 + UTR, Agilent Technologies) from different amounts of Covaris-sheared DNA using the KAPA Library Preparation Kit (green) or a competitor kit (blue). Optimally formulated and evolved enzymes, combined with a highly optimized “with-bead” protocol result in higher library yields with the KAPA Library Preparation Kit. This translates to higher library diversity and lower duplication rates, even with significantly less input DNA. Paired-end sequencing (2 x 100 bp) was performed on an Illumina HiSeq 2000. Data courtesy of the Translational Genomics Research Institute. Data on file.
Key sequencing metrics (library diversity, left and duplication rates, right) for libraries prepared for exome capture (SureSelect All Human Exome V5 + UTR, Agilent Technologies) from different amounts of Covaris-sheared DNA using the KAPA Library Preparation Kit (green) or a competitor kit (blue). Optimally formulated and evolved enzymes, combined with a highly optimized “with-bead” protocol result in higher library yields with the KAPA Library Preparation Kit. This translates to higher library diversity and lower duplication rates, even with significantly less input DNA. Paired-end sequencing (2 x 100 bp) was performed on an Illumina HiSeq 2000. Data courtesy of the Translational Genomics Research Institute. Data on file.
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Achievegreatermolecularcomplexity

  • Higheryieldsofadapter-ligatedlibrarytranslatestohighermolecularcomplexity
  • Fewercyclesofamplificationareneeded,whichresultsinlowerPCRduplicationrates
  • PCR-freeworkflowspossIBLefrominputs≥100ng*
Indexed libraries were constructed and amplified from 100 ng human genomic DNA using either the KAPA Library Preparation Kit (right) or the standard library preparation reagents and protocol in use at The Broad Institute (left). Individual libraries were quantified using qPCR, pooled prior to denaturation, and sequenced (pair-ended, 2 x 25 bp) on an Illumina HiSeq 2000. Libraries prepared using the KAPA Library Preparation Kit resulted in more uniform coverage distribution across the range of GC-content. Data courtesy of The Broad Institute. Data on file.
Indexed libraries were constructed and amplified from 100 ng human genomic DNA using either the KAPA Library Preparation Kit (right) or the standard library preparation reagents and protocol in use at The Broad Institute (left). Individual libraries were quantified using qPCR, pooled prior to denaturation, and sequenced (pair-ended, 2 x 25 bp) on an Illumina HiSeq 2000. Libraries prepared using the KAPA Library Preparation Kit resulted in more uniform coverage distribution across the range of GC-content. Data courtesy of The Broad Institute. Data on file.
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Reduceamplificationbiasandimprovecoverage

  • KAPAHiFireducesPCRbias*
  • ImprovescoverageuniformityofGC-andAT-richregions,promoters,lowcomplexityandotherchallengingregions*
Key sequencing metrics (fraction of duplicates, left and post-capture library complexity, right) for FFPE libraries prepared with the KAPA Library Preparation Kit for target capture (SeqCap EZ custom panel, 279 genes, 1 MB; Roche Nimblegen). Of each of the eight colon/gastric cancer FFPE samples, 250 ng FFPE DNA was Covaris-sheared for automated library construction, using a Biomek FXp instrument (Beckman Coulter). KAPA HiFi HotStart ReadyMix was used for both pre- and post-capture amplification (8 and 12 cycles, respectively). Paired-end sequencing (2 x 75 bp) was performed on an Illumina MiSeq. Data courtesy of Memorial Sloane-Kettering Cancer Center. Data on file.
Key sequencing metrics (fraction of duplicates, left and post-capture library complexity, right) for FFPE libraries prepared with the KAPA Library Preparation Kit for target capture (SeqCap EZ custom panel, 279 genes, 1 MB; Roche Nimblegen). Of each of the eight colon/gastric cancer FFPE samples, 250 ng FFPE DNA was Covaris-sheared for automated library construction, using a Biomek FXp instrument (Beckman Coulter). KAPA HiFi HotStart ReadyMix was used for both pre- and post-capture amplification (8 and 12 cycles, respectively). Paired-end sequencing (2 x 75 bp) was performed on an Illumina MiSeq. Data courtesy of Memorial Sloane-Kettering Cancer Center. Data on file.
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Createhigh-qualitylibrariesfromchallengingsamples

  • High-qualitywholeexomesequencingfromlibrariespreparedusingaslittleas10nghumangenomicDNA*
  • Highsuccessrateswith250ngFFPEorless*
  • Routinelibraryconstructionfrom≥100pgChIPDNA*
KAPA Library Quantification methods (96- and 384-well) format are available for the Sciclone NGS and NGSx workstation (PerkinElmer) and other instruments used in high-throughput NGS sample preparation pipelines.

Automatewithpre-validatedscripts

 

*Dataonfile.

Applications:

Applications
  • WholeGenomeSequencing
  • ChIPSequencing
  • WholeExomeSequencing
  • AmpliconSequencing
  • TargetedSequencing(Capture)
  • MethylSequencing

KitSpecificationsandContents/Storage:

KitSpecificationsandContents/Storage

Kitscanbestoredforupto18months at-20˚C.

Kitsincludereagentsforendrepair,A-tailing,ligationandlibraryamplification.PCR-freeversions(withoutlibraryamplificationreagent)arealsoavailable.“With-bead”kitsincludePEG/NaClsolutionforSPRIbeadcleanups.
All96-librarykitsareautomationfriendly.

Components

Specifications

Spec
Description
CompatiblePlatform
IlluminaHiSeq,MiSeq,NextSeqandGAIIx
LibraryType
DNA
StartingMaterial
FragmentedDNAorPCRamplicons
InputAmount
100pg–5µg
SequencingApplications
WholeGenomeSequencingWholeExomeSequencingTargetedSequencing(custompanels)ChIP-SeqAmpliconSequencingMethyl-Seq
Kapa biosystems表观遗传分析基于阵列的方法和基于下一代测序（NGS）的方法都用于研究表观遗传修饰。有三种基于NGS的常见方法可用于表观遗传分析：甲基序列，ChIP序列和ATAC序列。Methyl-seq   用单核苷酸分辨率研究基因组的甲基化状态。该方法采用亚硫酸氢盐处理，可将胞嘧啶残基转化为尿嘧啶，而甲基化残基则保持不变。已经开发了几种甲基序列策略，包括全基因组亚硫酸氢盐测序（WGBS）和简化表示的亚硫酸氢盐测序（RRBS），这丰富了CpG岛。ChIP-seq将染色质免疫沉淀（ChIP）与NGS结合使用，以鉴定整个基因组中与DNA相关的蛋白质的结合位点，通常用于绘制组蛋白修饰和转录因子。该方法依靠靶向抗体的选择来富集与特定蛋白质结合的目标DNA片段。ATAC-seq是用于转座酶可及的染色质测序的一种测定方法，可确定染色质可及性的区域并绘制DNA结合蛋白的图谱，以鉴定活性启动子，增强子和其他顺式调控元件。该方法通过生成具有50,000个细胞的测序文库，已经改变了基因调控的分析方法。由于表观遗传学分析通常涉及超低输入DNA，因此从有限的材料构建高质量文库至关重要。罗氏测序解决方案提供了许多用于表观遗传工作流程的目标富集，文库制备和优化文库质量的解决方案。SeqCap®Epi靶标富集试剂盒可通过单碱基分辨率富集用于DNA甲基化评估的靶标。的  KAPA HyperPrep套件  理想地适合于这两个芯片起和甲基SEQ应用，因为它使接头连接的文库和扩增低偏压的更高的产率。这意味着更高的文库多样性，更低的重复率和更统一的覆盖率，尤其是对于低输入样本而言。对于甲基序列研究，  KAPA HiFi Uracil + 由于对尿嘧啶残基具有耐受性，HotStart DNA聚合酶对于亚硫酸氢盐转化的文库的扩增至关重要。KAPA HiFi HotStart ReadyMix可用于扩增ATAC-seq和ChIP-seq库，从而改善序列覆盖范围并减少偏差。