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蚂蚁淘在线 / 品牌中心 / Kapa biosystems / Kapa biosystems/KAPA Hyper Prep Kits/KK8702/12 adapters x 40 µl each

Kapa biosystems/KAPA Hyper Prep Kits/KK8702/12 adapters x 40 µl each

价格
￥18720.00
货号：KK8702
浏览量：127
品牌：Kapa biosystems
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商品描述

Description:

KAPASingle-IndexedAdapterKit,SetB(30µM)

KAPAHyperPrepKits

Shiftyourworkflowintohyperdrive.

TheKAPAHyperPrepKitisaversatile,streamlinedsolutionforDNAlibrarypreparationforIllumina®sequencing.

Thenovelone-tubechemistry,containoptimallyformulatedandevolvedenzymesthatenablehigheryieldsofadapter-ligatedlibraryandloweramplificationbias.Thistranslatestohigherlibrarydiversity,lowerduplicationratesandmoreuniformcoverage,particularlyforFFPEandlow-inputsamples.

NEW!KAPADual-IndexedAdapterKitsarenowavailable.FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapterandBeadCalculator.

DownloadourAdapterandBeadCalculator

 

ForResearchUseOnly.Notforuseindiagnosticprocedures.

ProductHighlights

KAPA Hyper Prep Kits convert more input DNA to adapter-ligated library. Libraries were prepared from Covaris-sheared DNA and quantified prior to library amplification with the KAPA Library Quantification Kit. The KAPA Hyper Prep Kits achieved the highest yield of adapter-ligated library across the range of inputs (1 μg, 100 ng or 10 ng), and required fewer cycles of amplification to generate 1 μg of amplified library required for target capture. Data on file.
Number of amplification cycles needed to generate ≥1 µg amplified library for target capture with the SeqCap EZ Comprehensive Cancer Design (578 genes, 4 MB; Roche Nimblegen), for libraries prepared with each kit. The higher conversion rates achieved with the KAPA Hyper Prep Kit, combined with high-efficiency, low-bias amplification with KAPA HiFi DNA Polymerase, translates to significantly less cycles of pre-capture amplification and subsequent improvements in sequence data quality. Data on file.
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Improvelibraryyieldsandsequencequality

  • Higherlibraryyieldstranslatetogreatermolecularcomplexity
  • FewercyclesofamplificationwithKAPAHiFiDNAPolymeraseresultsinlowerduplicationratesandimprovedcoverage
Sequencing metrics FFPE libraries prepared for whole exome sequencing. Libraries were prepared from 100 ng of FFPE DNA, and exome capture was performed with the SeqCap EZ HGSC VCRome Design, Roche NimbleGen. Higher library yields enabled fewer cycles of amplification, leading to significantly lower duplication rates and higher target coverage. Data on file.
Conversion rates for FFPE DNA has been identified as a primary bottleneck in NGS library construction. With the KAPA Hyper Prep Kit, conversion rates for FFPE samples are similar to those for high-quality DNA. This leads to significantly lower duplication rates and higher overall target coverage in exome and other target capture workflows. Conversion data are for libraries prepared from 80 – 100 ng. Covaris-sheared FFPE DNA of variable quality, or 100 ng control DNA. Data on file.
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Createhigh-qualitylibrariesfromFFPEsamples

  • Generatehigh-qualitylibrariesfrom250ngFFPEDNAorless*
  • Significantlylowerduplicationratesandhighercoverage*
The streamlined KAPA Hyper Prep workflow. End repair, A-tailing and ligation is performed in a single tube, without intervening cleanup steps. The workflow is ideally suited for robust, high-throughput sample preparation for Illumina sequencing. Data on file.

Completelibraryconstructioninlessthan3hours

  • One-tubeworkflowwithminimalcleanupstepsreducesoveralltime,andminimizeshands-ontime
  • Sample-to-libraryin<2hoursforPCR-freeworkflows,or<3hourswithamplification*
  • Fewerhandlingstepsleadtoimprovedconsistencyandreproducibility
Key sequencing metrics for libraries prepared from 2 or 10 ng free-circulating DNA from plasma, using the KAPA Hyper Prep Kit or KAPA LTP Library Preparation Kit, with different adapter:insert molar ratios. Libraries were amplified with the Real-Time library Amplification Kit, prior to sequencing on an Illumina MiSeq. With the novel KAPA Hyper Prep chemistry, higher adapter concentrations can be used to improve library diversity. Data on file.
Key sequencing metrics for libraries prepared from 2 or 10 ng free-circulating DNA from plasma, using the KAPA Hyper Prep Kit or KAPA LTP Library Preparation Kit, with different adapter:insert molar ratios. Libraries were amplified with the Real-Time library Amplification Kit, prior to sequencing on an Illumina MiSeq. With the novel KAPA Hyper Prep chemistry, higher adapter concentrations can be used to reduce duplication rates. Data on file.
Key sequencing metrics for libraries prepared from 2 or 10 ng free-circulating DNA from plasma, using the KAPA Hyper Prep Kit or KAPA LTP Library Preparation Kit, with different adapter:insert molar ratios. Libraries prepared with the two kits had similar insert sizes (D). Data on file.
Key sequencing metrics for libraries prepared from 2 or 10 ng free-circulating DNA from plasma, using the KAPA Hyper Prep Kit or KAPA LTP Library Preparation Kit, with different adapter:insert molar ratios. Libraries prepared with the two kits had similar insert sizes (D). Data on file.
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Improveperformancewithlow-inputsamples

  • Generatemoresequence-qualitylibrarymoleculeswithoutincreasingadapter-dimers
  • Increaseyieldswithoutinducingsizebias

 

*Dataonfile.

RelatedProducts

Areyousequencinglow-input,FFPEorhighqualityDNA? RNA? CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:

Sample QC

KAPAhgDNAQuantificationandQCKit

Library Amplification

KAPALibraryAmplificationKits

Library Quantification

KAPALibraryQuantificationKits

KAPA Stranded mRNA-Seq Kits

KAPAStrandedmRNA-SeqKits

Applications:

Applications
  • WholeGenomeSequencing
  • WholeExomeSequencing
  • TargetedSequencing(custompanels)
  • ChIP-seq
  • AmpliconSequencing
  • Methyl-seq

KitSpecificationsandContents/Storage:

KitSpecificationsandContents/Storage

Kitscanbestoredforupto12months at-20˚C.

Kitscontain KAPAEndRepair&A-TailingBuffer, KAPAEndRepair&A-TailingEnzyme, KAPALigationBuffer, KAPADNALigase, KAPAHiFiHotStartReadyMixand KAPALibraryAmplificationPrimerMix

Components

Hyper_Component Chart

Specifications

Spec
Description
CompatIBLePlatform
lluminaHiSeq,MiSeq,NextSeqandGAIIx
LibraryType
DNA
StartingMaterial
FragmentedDNA,CDNA,orPCRamplicons
InputAmount
1ng–1µg
Kapa biosystems表观遗传分析基于阵列的方法和基于下一代测序（NGS）的方法都用于研究表观遗传修饰。有三种基于NGS的常见方法可用于表观遗传分析：甲基序列，ChIP序列和ATAC序列。Methyl-seq   用单核苷酸分辨率研究基因组的甲基化状态。该方法采用亚硫酸氢盐处理，可将胞嘧啶残基转化为尿嘧啶，而甲基化残基则保持不变。已经开发了几种甲基序列策略，包括全基因组亚硫酸氢盐测序（WGBS）和简化表示的亚硫酸氢盐测序（RRBS），这丰富了CpG岛。ChIP-seq将染色质免疫沉淀（ChIP）与NGS结合使用，以鉴定整个基因组中与DNA相关的蛋白质的结合位点，通常用于绘制组蛋白修饰和转录因子。该方法依靠靶向抗体的选择来富集与特定蛋白质结合的目标DNA片段。ATAC-seq是用于转座酶可及的染色质测序的一种测定方法，可确定染色质可及性的区域并绘制DNA结合蛋白的图谱，以鉴定活性启动子，增强子和其他顺式调控元件。该方法通过生成具有50,000个细胞的测序文库，已经改变了基因调控的分析方法。由于表观遗传学分析通常涉及超低输入DNA，因此从有限的材料构建高质量文库至关重要。罗氏测序解决方案提供了许多用于表观遗传工作流程的目标富集，文库制备和优化文库质量的解决方案。SeqCap®Epi靶标富集试剂盒可通过单碱基分辨率富集用于DNA甲基化评估的靶标。的  KAPA HyperPrep套件  理想地适合于这两个芯片起和甲基SEQ应用，因为它使接头连接的文库和扩增低偏压的更高的产率。这意味着更高的文库多样性，更低的重复率和更统一的覆盖率，尤其是对于低输入样本而言。对于甲基序列研究，  KAPA HiFi Uracil + 由于对尿嘧啶残基具有耐受性，HotStart DNA聚合酶对于亚硫酸氢盐转化的文库的扩增至关重要。KAPA HiFi HotStart ReadyMix可用于扩增ATAC-seq和ChIP-seq库，从而改善序列覆盖范围并减少偏差。