ForimagingwiththeLeicaTCSSP8CARSconfocalmicroscope,specimendonothavetobestained.TheCoherentanti-StokesRamanScattering(CARS)technologyinvolvestwolaserbeamstovisualizethevibrationalcontrastofmoleculesinspecimens.Thespecimenremainsalmostunaffected.
TheuserprofitsofawiderangeoflivecellimagingwiththecombinationofvisIBLe,IRandUVlasers,secondharmonicgenerationandCARSsuchasanalyzinglivingcells,tissuesandevensmallwholeanimalsathighspeedorathighresolution.
Withitsflexibilityandconvenienthandling,theLeicaTCSSP8CARSistheidealdeviceforresearchinstitutesandmulti-userfacilities.
Imagingofnativespecimenwithoutdyes
Theintegrationofthelabel-freeCARStechnologyintotheLeicaTCSSP8confocalplatformovercomesthelimitationofdye-basedimagingmethods.Imagingofthecharacteristicintrinsicvibrationalcontrastofthepuremoleculesinthesampledoesnotrequireanystaining.Thismethodisnon-toxic,non-destructiveandminimallyinvasive.
MultispectralimagingwithVIS,UV,IR,SHGandCARS
TheLeicaTCSSP8CARSisaplatformprovidingvariousimagingmethodssuchasCARS,singlephotonormultiphotonfluorescenceorforimagingwithUVlasersinsimultaneousorsequentialmode.
Lasersources,scannermodelandimagingmodecanbeoptimallyadaptedtotheapplicationofinterestandsetupthroughthesystemsoftwareLeicaLASAF.Theergonomicandintuitivehandlingensuresthatambitiousexperimentsrunsmoothly.
Easyexperimentsetupwithafullyintegratedsystem
ThecompactCARSlaserisfullycontrolledthroughthesystemsoftwareLASAF.Thetightintegrationofthelasercontrolintothesoftwaremakesthesophisticatedprocessofadjustingmultipleparametersofthesystemcompletelytransparent.ThissolutionoffersthemostconvenientwaytoperformCARSexperiments–forexpertsandnovicesalike.
Videorateandhighresolutioninonesystem
LeicaTCSSP8CARSincludestheLeicaTandemScanner.Itcomprisesaconventionalscannerforallmorphologyandclassicalspeedlivecellapplicationsandaresonantscannerthatprovidesawiderangeofhighspeedoptions.ThiscombinationoftwoscannerscoversamultitudeofapplicationsandmakestheLeicaTCSSP8CARSaflexibledevicefittedformulti-userfacilities.
