| productinformation
| | Background | | Assimilatorynitratereductase(NR),(EC.1.6.6.1)catalysesthereductionofnitratetonitriteinthecytoplasm.Plantscontain2formsofNR:NADH-NR(mostcommonforminplantsandalgae,predominantlyfoundingreentissues)andNAD(P)H-NR(usesNADHorNADPHastheelectrondonor,constitutivelyexpressedinplantsatalowlevel).NADH-NRisahomodimeroftwoidenticalsubunits(100-115kDaeach,holdtogetherbyaMo-cofactor)eachofthemcodedbyuptothreegenes(NR1-3,NIA1-NIA3). | | Immunogen | | KLH-conjugatedsyntheticpeptidederivedfromconserveddomaininNADH-NRproteinsequencesincludingA.thalianaNR1P11832,At1g77760andNR2P11035,At1g37130 | | Host | | Rabbit | | Clonality | | Polyclonal | | Clone | | | | Purity | | Affinitypurifiedserum | | Format | | Lyophilized | | Quantity | | 2x50µg | | Reconstitution | | Forreconstitutionadd25µlofsterilewatertoeachtube. | | Storage | | storelyophilized/reconstitutedat-20°C;oncereconstitutedmakealiquotstoavoidrepeatedfreeze-thawcycles.Please,remembertospintubesbrieflypriortoopeningthemtoavoidanylossesthatmightoccurfromlyophilizedmaterialadheringtothecaporsidesofthetubes. | | Testedapplications | | Westernblot(WB) | | Relatedproducts | | AS122611|NRT1.1|NITRATETRANSPORTER1.1,rabbitantibody
AS122612|NRT2.1|NITRATETRANSPORTER2.1,rabbitantibody
AS09473|NRT1.4|nitratetransporter,rabbitantibody Plantproteinextractionbuffer Secondaryantibodies | | Additionalinformation | | |
applicationinformation
| | Recommendeddilution | | 1:500-1:1000(WB) | | Expected|apparentMW | | 103kDa|117kDa | | Confirmedreactivity | | ArABIdopsisthaliana,Hordeumvulgare,Chlamydomonasreinardtii,redalgaGracilariagracilis,diatomThalassiosirasp.,PhaeodactylumtricornutumBohlinaccessionPt18.6,PopulusyunanensisDode,Solanumtuberosum,VignarADIata
| | Predictedreactivity | | dicotsincluding:Glycinemax,Glycinesoja, Lycopersicumesculentum,Nicotianatabacum,Nicotianaattenuata,Nicotianabenthamiana, Ricinuscommunis, Spinaciaoleracea,Solanumlycopersicum, Phaseolusvulgaris, Capsellarubella monocotsincluding:Oryzasativa,Zeamays,moss:Physcomitrellapatens; Chlorellavulgaris,Dunaliellasalina,marinediatoms
Eucalyptusgrandis,Coffeacanephora,Brassicanapus, Brassicarapasubsp.pekinensis,Vitisvinifera,Arabisalpina,Citrussinensis,Theobromacacao,Citrusclementina,Gossypiumarboretum,Prunuspersica,Morusalba,Sorghumbicolor,Spinaciaoleracea,brachypodiumdistachyon.
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